Human β ngf with enzyme linked immunosorbent assay elisa kit

PLGA (LA:GA = 50:50) and PDLLA with molecular weight of 100 kDa (as indicated by their inherent viscosity 0.6–0.8 dL/g) were purchased from Lakeshore Biomaterials, USA. Chloroform was supplied by Uni Chem Co., Korea. The human β-NGF with Enzyme Linked Immunosorbent Assay (ELISA) Kit, human GDNF with ELISA Kit were purchased from Peprotech Inc. and R&D Systems, Inc., respectively. Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), and trypsin/EDTA were purchased from Invitrogen, Inc., USA. Mouse anti-neurofilament (M + H) primary antibody, goat anti-mouse IgG secondary antibody (Alexa Fluor^® 594), phalloidin (Alexa Fluor^® 488), and DAPI (4’, 6-diamidino-2-phenylindole) were purchased from Life Technologies. Triton X-100, Span-80, phosphate buffered saline (PBS) tablets, heparin, bovine serum albumin (BSA), paraformaldehyde, glutaraldehyde, sodium cacodylate, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), and sucrose were Sigma-Aldrich products. Other chemicals were used as received.

PLGA (LA:GA=50:50) and PDLLA with molecular weight of 100 kDa (as indicated by their inherent viscosity 0.6–0.8 dL/g) were purchased from Lakeshore Biomaterials, USA. Chloroform was supplied by Uni Chem Co., Korea. The human β-NGF with Enzyme Linked Immunosorbent Assay (ELISA) Kit, human GDNF with ELISA Kit were purchased from Peprotech Inc. and R&D Systems, Inc., respectively. Span-80, phosphate buffered saline (PBS) tablets, heparin and bovine serum albumin (BSA) were Sigma-Aldrich products. Other chemicals were used as received.