Anti rabbit hrp a 6154

Primary Antibodies: Anti TUBB4B (WB-1:500; IF, IHC-1:200), Ephrin-B1(WB-1:500; IF, IHC-1:100) and Ephrin-B1-Alexa680(IF, IHC-1:50) were from Santa Cruz Biotechnology (Dallas, Texas, USA); GAPDH (WB-1:1000) antibody was from Thermo Scientific, USA and Mouse IgG isotype control, Rabbit IgG isotype control and Na⁺K⁺-ATPase (1:1000) was from Cell Signaling Technologies (Danvers, Massachusetts, USA); ALDH1A1(IF, IHC-1:50) and Veri-blot IP detection antibody(1:200) were from Abcam (Cambridge, United Kingdom).
Secondary antibodies: Anti-Mouse HRP (A-3673) (1:5000), Anti-Rabbit HRP (A-6154) (1:5000) were procured from Sigma-Aldrich (St. Louis, Missouri, USA). Anti-Mouse Alexa Fluor 488 (1:500), Anti-Mouse Alexa Fluor 568 (1:500) were from Invitrogen.

All primary and secondary antibodies for immunoblotting were used at 1:1000 and 1:2000 dilutions, respectively. The following reagents were used for the experiments in this study: anti-O-linked-N-acetylglucosamine antibody (RL2) (MA1-072) and anti-OGT (PA5-22071) were from Thermo Fisher Scientific; anti-b-actin (A2066), anti-rabbit-HRP (A6154), and anti-mouse-HRP (A4416) were from Sigma-Aldrich; anti-p21 (2947S), anti-p27 (3686S), and anti-Skp2 (4358S) were from Cell Signaling; and anti-FoxM1 (sc-502) was from Santa Cruz. TMG (SML-0244), OSMI-1 (SML-162), and cycloheximide (C4859) were purchased from Sigma-Aldrich. siRNAs against OGT #7 (SI02665131) and #8 (SI04713604) were purchased from Qiagen, and siRNA #1 (SI6094) and siCtrl siRNA (4390843) were purchased from Ambion. A FoxM1 overexpression plasmid (sc-128214) and empty vector control plasmid were purchased from OriGene. Transfection of plasmids was performed using Mirus TransIT X2 transfection reagent, and siRNAs were transfected using Lipofectamine RNAiMax (Thermo Fisher).