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Hmvec c

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HMVEC-C is a primary human microvascular endothelial cell line derived from the cardiac tissue. It serves as a model for the study of cardiac microvascular endothelial cell biology.

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Lab products found in correlation

Egm 2 medium, by Lonza (4 mentions) Human umbilical vein endothelial cells (huvec), by Lonza (2 mentions) Lsm 800 laser scanning microscope, by Zeiss (2 mentions) Egm 2mv medium, by Lonza (1 mentions) Human umbilical vein endothelial cells (huvec), by Cambrex (1 mentions) Egm 2 singlequot, by Lonza (1 mentions) Matrigel, by Corning (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) F1141, by Merck Group (1 mentions) Cc 7030, by Lonza (1 mentions) Hcaec, by Lonza (1 mentions) Hmvec d, by Lonza (1 mentions) Hmvec l, by Lonza (1 mentions) Fibroblast growth factor 2 (fgf2), by Merck Group (1 mentions) Hcmec d3, by Merck Group (1 mentions) Egm 2mv bulletkit, by Cambrex (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Lonza (1 mentions) Primary human umbilical vein endothelial cells, by Lonza (1 mentions) Penicillin streptomycin amphotericin b, by Lonza (1 mentions)

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HMVECs (86 citations)

13 protocols using hmvec c

1

Isolation and Culture of Endothelial Cells

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HUVEC were obtained from umbilical cord vein by collagenase digestion as described (Jaffe et al. 1973 (link)) and grown to the fourth passage in EGM-2 medium (Lonza, France) (containing 2% fetal bovine serum), under standard cell culture conditions (humidified atmosphere at 37°C, 5% CO2). Human aortic endothelial cells (HAoEC) and cardiac-derived microvascular endothelial cells (HMVEC-C) were obtained from Lonza. HMVEC-C were grown to the fifth passages in EGM-2 MV medium (Lonza, France) (containing 5% fetal bovine serum) under standard cell culture conditions. HAoEC were grown to the fifth passage in EGM-2 medium under standard cell culture conditions.
Addi T., Poitevin S., McKay N., El Mecherfi K.E., Kheroua O., Jourde-Chiche N., de Macedo A., Gondouin B., Cerini C., Brunet P., Dignat-George F., Burtey S, & Dou L. (2019). Mechanisms of tissue factor induction by the uremic toxin indole-3 acetic acid through aryl hydrocarbon receptor/nuclear factor-kappa B signaling pathway in human endothelial cells. Archives of toxicology, 93(1).
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2

Endothelial Cell Culture Protocol

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Human umbilical vein endothelial cells (Cambrex Bio Science Inc, Walkersville, MD, USA) and human cardiac microvascular endothelial cells (HMVEC-C, Lonza, Milan, Italy) were cultured in endothelial growth medium (EGM-2 medium from Lonza) at 37°C in a humidified atmosphere of 5% CO2. Cells were exposed to the culture medium with 10% foetal bovine serum for at least 1 day before experiments. All assays were conducted using low cell passage cells (2–5 passages).
Agostini S., Chiavacci E., Matteucci M., Torelli M., Pitto L, & Lionetti V. (2014). Barley beta-glucan promotes MnSOD expression and enhances angiogenesis under oxidative microenvironment. Journal of Cellular and Molecular Medicine, 19(1), 227-238.
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3

Cardiac Endothelial Cell Culture

Cited 1 time
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HMVECs of cardiac origin (HMVEC-C) were obtained from Lonza (cat# CC-7030). ECs were cultured and maintained in an EBM-2 basal medium supplemented with EGM-2 MV SingleQuots™ Kit (cat# CC-3156/CC-4147). Experiments were performed at P6, considering primary ECs may become senescent at passages P8–1067 (link).
Yepuri G., Ramirez L.M., Theophall G.G., Reverdatto S.V., Quadri N., Hasan S.N., Bu L., Thiagarajan D., Wilson R., Díez R.L., Gugger P.F., Mangar K., Narula N., Katz S.D., Zhou B., Li H., Stotland A.B., Gottlieb R.A., Schmidt A.M., Shekhtman A, & Ramasamy R. (2023). DIAPH1-MFN2 interaction regulates mitochondria-SR/ER contact and modulates ischemic/hypoxic stress. Nature Communications, 14, 6900.
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4

Culturing Human Endothelial Cells and Hepatocytes

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Human umbilical endothelial cells (HUVEC; Lonza) were maintained in EBM-2 media supplemented with EGM-2 SingleQuots (Lonza). Human cardiac microvascular endothelial cells (HMVEC-C; Lonza) were maintained in EBM-2 media supplemented with EGM-2V SingleQuots (Lonza). HepG2 hepatocarcinoma cells were purchased from ATCC and grown in modified eagle's medium (MEM) supplemented with 10% FBS, L-glutamine, and sodium pyruvate. Human recombinant brain-derived neurotrophic factor (BDNF) was purchased from Sigma-Aldrich and highly purified C-reactive protein (CRP), sodium azide and endotoxin free, was obtained from TriChem Resources Inc.
Noren Hooten N., Ejiogu N., Zonderman A.B, & Evans M.K. (2015). Protective Effects of BDNF against C-Reactive Protein-Induced Inflammation in Women. Mediators of Inflammation, 2015, 516783.
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5

Angiogenesis Assay of Cardiac Endothelial Cells

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Human microvascular endothelial cells (cardiac) (HMVEC-C) and microvascular endothelial cell growth medium-2 (EGM-2 MV) culture media plus bullet kit were purchased from Lonza. For the in vitro angiogenesis assay, Matrigel for tube formation was purchased from Corning (Corning, NY, USA). In vitro tube formation assays were carried out following manufacturer’s instruction. Briefly, HMVEC-Cs were added onto Matrigel reduced growth factor basement membrane extract in a 96-well plate. Four different types of conditioned media were collected from donor 1 cells at 24 hours in HMVEC-C basal media (EGM-2 MV media + 5 % fetal bovine serum). These were added into each well to observe tube formation of HMVEC-C in different culture conditions. EGM-2 MV complete culture media (EGM-2 MV media + bullet kit) was used as a positive control. Each datum point was averaged from 12 samples assayed on a Zeiss D1 Observer (Lonza, Walkersville, MD, USA) inverted microscope, and the images were blindly analyzed with the ImageJ Angiogenesis Analyzer (Zeiss Microscopy, Pleasanton, CA, USA; Image J, U. S. National Institutes of Health, Bethesda, Maryland, USA) for length of tubes formed, number of branches formed, number of junctions formed, and number of nodes formed.
Lin X., Robinson M., Petrie T., Spandler V., Boyd W.D, & Sondergaard C.S. (2015). Small intestinal submucosa-derived extracellular matrix bioscaffold significantly enhances angiogenic factor secretion from human mesenchymal stromal cells. Stem Cell Research & Therapy, 6(1), 164.
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6

Endothelial Cell Culture and Characterization

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Human Umbilical Vein Endothelial Cells (HUVEC, C2517A) and Human Cardiac Microvascular Endothelial Cells (HMVEC-C, CC7030) were purchased from Lonza and cultured in EGM-2 medium (Lonza) on collagen I (5 µg/cm2, Gibco, A10483) or fibronectin (1 µg/cm2, Sigma, F1141) pre-coated plates unless otherwise indicated. Cells obtained from Lonza were considered as passage 1, and only cells from passage 3 to 6 were used for the experiments. Cells were harvested for analysis at sub-confluent stage. E0771 murine breast cancer cells were purchased from CH3 Biosystems and cultured in RPMI-1640 medium (Gibco) with 10% fetal bovine serum (FBS, Gibco). Primary endothelial cells and E0771 cells were authenticated by the company. All cell stocks were tested for mycoplasma contamination by PCR and were negative.
Wang H., Lapek J., Fujimura K., Strnadel J., Liu B., Gonzalez D.J., Zhang W., Watson F., Yu V., Liu C., Melo C.M., Miller Y.I., Elliott K.C., Cheresh D.A, & Klemke R.L. (2018). Pseudopodium-enriched atypical kinase 1 mediates angiogenesis by modulating GATA2-dependent VEGFR2 transcription. Cell Discovery, 4, 26.
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7

Isolation and Culture of Primary Human Endothelial Cells

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Primary cultures of human endothelial cells derived from multiple vascular beds were used in this study. Human cerebral microvascular endothelial cells (HCMEC/D3) were purchased from Sigma-Aldrich (Sigma-Aldrich, Millipore, St. Louis, MO, USA), and cells were maintained following the manufacturer’s instructions. The cells were grown in cultured media of EndoGRO™-MV Complete Media Kit (Cat. No. SCME004, Sigma-Aldrich) supplemented with 1 ng/mL FGF-2 (Cat. No. GF003, Sigma-Aldrich). Human coronary artery endothelial cells (HCAEC), human iliac artery endothelial cells (HIAEC, Lonza, Walkersville, MD, USA), human dermal microvascular endothelial cells (hMVEC-d), human cardiac microvascular endothelial cells (HMVEC-C), and human lung microvascular endothelial cells (HMVEC-L) were purchased from Lonza (Walkersville, MD, USA), and cells were maintained following the manufacturer’s instructions. For cell culture, the procedures consisted in use of endothelial growth medium (EGM™-2MV BulletKit™; Clonetics) supplemented with 5% fetal bovine serum (FBS; Clonetics) and cells were incubated at 37 °C and 5% CO2. For serial passaging (always before passage 4), the cells were dissociated by trypsinization, centrifuged at 220× g for 7 min, and replated at the correct density.
Khalyfa A., Gozal D, & Kheirandish-Gozal L. (2019). Plasma Extracellular Vesicles in Children with OSA Disrupt Blood–Brain Barrier Integrity and Endothelial Cell Wound Healing In Vitro. International Journal of Molecular Sciences, 20(24), 6233.
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8

Culturing Diverse Cell Lines and Endothelial Cells

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The human A431 epithelial carcinoma cell line, 3T3 mouse fibroblast cell line and HT29 human colon carcinoma cell line were obtained from ATCC. Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Lonza) containing L-glutamine, supplemented with 10% Fetal Bovine Serum (Invitrogen) and Penicillin-Streptomycin-Amphotericin B (Lonza). Primary human umbilical vein endothelial cells (HUVEC) and primary human cardiac microvascular endothelial cells (HMVEC-C) were purchased from Lonza and cultured in EGM2 medium (Lonza) containing Penicillin-Streptomycin-Amphotericin B (Lonza).
Méhes E., Biri-Kovács B., Isai D.G., Gulyás M., Nyitray L, & Czirók A. (2019). Matrigel patterning reflects multicellular contractility. PLoS Computational Biology, 15(10), e1007431.
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9

Culturing Human Endothelial Cells

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Human umbilical vein endothelial cells (HUVEC) and human cardiac microvascular endothelial cells (HMVEC-c) were obtained from Lonza (Allendale, NJ) and grown in endothelial growth medium (EGM2MV; Lonza) on 1.5% porcine gelatin matrix (Sigma-Aldrich, St. Louis, MO) in a 37°C, 5% CO2 incubator. HUVEC were used at passages 1-4 and HMVEC-c at passages 4-8.
Doggett T.M, & Breslin J.W. (2014). Acute Alcohol Intoxication-Induced Microvascular Leakage. Alcoholism, clinical and experimental research, 38(9), 2414-2426.
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10

Uptake of Ac-SDKP by Human Cardiac Microvascular Endothelial Cells

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To examine the uptake of Ac-SDKP by Human Cardiac Microvascular Endothelial Cells (HMVEC-Cs; Cat No. CC-7030, Lonza), we first performed in vitro cell imaging studies using fluorescein isothiocyanate (FITC) conjugated with Ac-SDKP. The FITC-conjugated Ac-SDKP was synthesized using Fmoc solid phase peptide synthesis (Genescript, Piscataway, NJ) and FITC-conjugated scrambled peptide, Ac-KDPS was synthesized using similar Fmoc synthesis protocol (Lifetein, Somerset, NJ). For these studies, peptide uptake was examined in both irradiated and non-irradiated (control) HMVEC-C cells at four hours post peptide treatment. For confocal image analysis, HMVEC-C cells were cultured on sterile cover slips in a 12 well plate. One of the plates was exposed to 9 Gy of radiation using a specialized orthovoltage radiation chamber and a sub group of cells were incubated with FITC-labeled Ac-SDKP or scrambled peptides at 14 μM. Images were captured using a confocal microscope (ZEISS LSM 800 Laser scanning microscope) at 400X magnification. For determining the uptake using FACs, cells were detached, irradiated and incubated with FITC-labeled scrambled peptide or Ac-SDKP (0.9, 1.8, 3.5, 7 & 14 μM). Cell uptake percentage was examined with a BD Accuri C6-Plus flow cytometer and further analyzed by FCS express 6-De Novo Software.
Sharma U.C., Sonkawade S.D., Baird A., Chen M., Xu S., Sexton S., Singh A.K., Groman A., Turowski S.G., Spernyak J.A., Mahajan S.D, & Pokharel S. (2018). Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flow. Cardio-oncology, 4, 8.
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