Multi shaker psu 20

Drug content of the feedstock material used for 3D printing was measured in distilled water in order to evaluate the uniformity of the prepared hot-melt extruded filaments. Briefly, 150 ± 6 mg of hot-melt extruded filaments pieces, taken from random sections of the extruded strand, were placed in 100 mL of distilled water and shaken at 120 rpm (Multi-shaker PSU 20, Biosan, Latvia) for a minimum of 24 h. Samples were diluted and subsequently measured utilizing a UV-Vis spectrophotometer (Lambda 35, PerkinElmer, Singapore) at 263 nm. Ten replicates of each formulation were analyzed.
Drug content data for the 3D-printed tablets was obtained from the dissolution study. Concisely, once the dissolution for each tablet had reached a plateau around 100% drug release, the mean value of five subsequent time points was calculated and reported as the drug content (mean ± SD, n = 3). Since the drug release for formulation, 13 had not reached 100% for all the tablets after 24 h; the tablets were kept in the dissolution vessels for another 22 h in room temperature, and the absorbance was then measured manually.

For the determination of carotenoids, 2.5 g of the testing material was transferred to a mortar, and about 15 g of anhydrous Na₂SO₄, previously weighed on a technical balance, was added [18 (link)]. The content of the mortar was ground until a homogeneous mass was obtained. The grated mass was then quantitatively transferred to a 100 mL ground-glass conical flask using 25 mL of hexane. The flasks were capped, wrapped with aluminum foil to minimize their exposure to light, and mixed on a Biosan Multi-Shaker PSU 20 for 48 h. The extraction was performed analogously in yoghurts after prior deproteinization.