Labotora 4000eco

LNE was prepared as described previously [38 (link)]. Briefly, the dried leaves of L. nobilis were purchased from the Hub Village (Ochang, Korea) and extracted with 70% ethanol for 3 h at room temperature. The filtered extract was then evaporated using a rotary evaporator (LABOTORA 4000eco, Heidolph Instruments GmbH&Co., Schwabach, Germany) to remove the solvent. The lyophilized sample was stored at –20 °C and was used after dissolving in dimethyl sulfoxide (DMSO) before each experiment. The presence of eucalyptol in the extract was identified by gas chromatography–mass spectrum (GC–MS) chromatogram (Agilent 7890A, Santa Clara, CA, USA).

The dried C. atrati (CA) root was purchased from Daekwang Medicine Company (Chuncheon, Korea), and the extraction procedure was followed as in the previous report [22 (link)]. Briefly, CA was powdered and extracted in 70% ethanol at room temperature for 3 h. The filtered extract was evaporated to remove solvent (LABOTORA 4000eco, Heidolph Instruments GmbH&Co., Schwabach, Germany) and stored in a freezer at −20 °C until used.
For the compositional analysis of CA, sample solution was subjected to ultrahigh pressure liquid chromatography system (UHPLC) using an LTQ Orbitrap XL linear ion trap mass spectrometry (MS) system (Thermo Scientific, San Jose, CA, USA) equipped with an electrospray ionization source. Chromatographic separation was performed using an ACQUITY UPLC^® BEH C₁₈ column (2.1 × 150 mm, 1.7 μm; Waters, Milford, MA, USA) and operated using mobile phases A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile). Each compound was detected with photodiode array at 200~500 nm. The Orbitrap analyzer was used for high-resolution and accurate mass (HR/AM) data acquisition with 30,000 FWHM resolving power at 400 m/z. The MS/MS experiments were adjusted using the Xcalibur system (Thermo Scientific, San Jose, CA, USA) and performed under automatic gain control (AGC) conditions.