Preparation of protein extracts and Western blot analysis were performed as described previously19 (link). Anti-GFP monoclonal antibody JL-8 (Clontech), anti-phospho-p38 MAPK monoclonal antibody D3F9 (Cell Signaling), anti-Hog1 polyclonal antibody y-215 (Santa Cruz), anti-phospho-p44/42 MAPK polyclonal antibody (Cell Signaling), anti-Mpk1 polyclonal antibody yN-19 (Santa Cruz), and anti-Mcm2 polyclonal antibody N-19 (Santa Cruz) were used. Detection was carried out by using a LAS-4000 (Fuji Film) with Immobilon Westren (Merck Millipore). Signal intensities were quantified by ImageQuant (GE Healthcare), and statistical analysis was performed with Excel (Microsoft).
Anti phospho p38 mapk monoclonal antibody d3f9
Manufactured by Cell Signaling Technology
The Anti-phospho-p38 MAPK monoclonal antibody D3F9 is a laboratory reagent used to detect the phosphorylated form of the p38 mitogen-activated protein kinase (MAPK) protein. It is a specific and sensitive tool for analyzing the activation of the p38 MAPK signaling pathway in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti mpk1 polyclonal antibody yn 19, by Santa Cruz Biotechnology (2 mentions)
Anti phospho p44 42 mapk polyclonal antibody, by Cell Signaling Technology (2 mentions)
Anti hog1 polyclonal antibody y 215, by Santa Cruz Biotechnology (2 mentions)
Anti mcm2 polyclonal antibody n 19, by Santa Cruz Biotechnology (2 mentions)
Las 4000, by Fujifilm (2 mentions)
Anti gfp monoclonal antibody jl 8, by Takara Bio (1 mentions)
Immobilon westren, by Merck Group (1 mentions)
Imagequant, by GE Healthcare (1 mentions)
Immobilon western, by Merck Group (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Anti myc monoclonal antibody 9e10, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti phospho p38 mapk monoclonal antibody d3f9
1
Quantitative Protein Analysis by Western Blot
2
Western Blot Protein Analysis Workflow
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Preparation of protein extracts and Western blot analysis were performed as described previously9 (link). Anti-phospho-p38 MAPK monoclonal antibody D3F9 (Cell Signaling), anti-Hog1 polyclonal antibody y-215 (Santa Cruz), anti-phospho-p44/42 MAPK polyclonal antibody (Cell Signaling), anti-Mpk1 polyclonal antibody yN-19 (Santa Cruz), anti-Myc monoclonal antibody 9E10 (Santa Cruz) and anti-Mcm2 polyclonal antibody N-19 (Santa Cruz) were used. Detection was carried out by using a LAS-4000 (Fuji Film) with Immobilon Western (Merck Millipore) or the Odyssey Imaging Systems (LI-COR Biosciences). Signal intensities were quantified by the Odyssey Imaging Systems, and statistical analysis was performed with Excel (Microsoft).
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