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Fibronectin

Manufactured by Novoprotein
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Sourced in China

Fibronectin is a high-molecular-weight glycoprotein found in the extracellular matrix and on the surface of various cell types. It plays a crucial role in cell adhesion, migration, and wound healing processes.

Automatically generated - may contain errors

Lab products found in correlation

Lipid concentrate, by Thermo Fisher Scientific (1 mentions) Hydrocortisone, by Selleck Chemicals (1 mentions) Heparin sodium, by Selleck Chemicals (1 mentions) Putrescine, by Merck Group (1 mentions) Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Pdgf bb, by Thermo Fisher Scientific (1 mentions) Nahco3, by Thermo Fisher Scientific (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Pdgf ab, by Thermo Fisher Scientific (1 mentions) Vitamin c, by Merck Group (1 mentions)

2 protocols using fibronectin

1

Defined Culture Cocktail for Cell Expansion

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Besides using DMEM/F12 (Gibco) as a basal medium, other 13 kinds of chemically defined cocktail factors (13F) included 1× Insulin-Transferrin-Selenium (ITS; Gibco, 41400-045), 0.1% lipid concentrate (Gibco, 11905-031), 2 ng/mL TGF-β1 (Peprotech, AF-100-21C), 5 ng/mL bFGF (Peprotech, 100-18B), 10 ng/mL PDGF-AB (Peprotech, 100-00AB), 10 ng/mL PDGF-BB (Peprotech, 100-14B), 1 μg/mL fibronectin (Novoprotein, CH38), 197.6 µM vitamin C (Sigma, A8960), 55.9 μM putrescine (Sigma, P5780), 17.8 nM progesterone (Selleck, S1705), 100 nM hydrocortisone (Selleck, S1696), 10 μg/mL heparin sodium (Selleck, S1346), and 1.722 g/L sodium bicarbonate (NaHCO3, Gibco, 25080-094).
Yi J., Zhang J., Zhang Q., Chen X., Qi R., Liang R., Wang Y., Wang F., Zhong Y., Zhang X., Chin G., Liu Q., Zhou W., Liu H., Chen J, & Ouyang H. (2022). Chemical-Empowered Human Adipose-Derived Stem Cells with Lower Immunogenicity and Enhanced Pro-angiogenic Ability Promote Fast Tissue Regeneration. Stem Cells Translational Medicine, 11(5), 552-565.
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2

Substrate Specificity of Rv2569c

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To evaluate the substrate specificity of Rv2569c protein, 4 μg of various substrates, including fibrinogen (Novoprotein, Suzhou, China), fibronectin (Novoprotein), and E-cadherin (Novoprotein), was incubated with 1 μg of Rv2569c purified protein in 20 μL of reaction volume (20 mM Tris-HCl, pH 8.0) for 12 h at 37°C. Then, the reaction products were examined by SDS-PAGE.
Zang X., Zhang J., Jiang Y., Feng T., Cui Y., Wang H., Cui Z., Dang G, & Liu S. (2024). Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin. PLOS Pathogens, 20(5), e1012214.
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