Horseradish peroxidase hrp conjugated β actin mouse monoclonal antibody

Forskolin and melatonin were purchased from Sigma-Aldrich. Y-27632 and arachidonic acid (AA) were purchased from Selleck Chemicals. The following antibodies were used in this study: BMAL1 (Abcam ab3350 for immunofluorescence and Santa Cruz Biotechnology sc-365645 for western blot), aggrecan (Millipore Sigma AB1031), MMP13 (Abcam ab39012 for western blot and Proteintech 18165-1-AP for immunofluorescence), and phosphomyosin light chain 2 (Ser19; Cell Signaling Technology 3671). Horseradish peroxidase (HRP)-conjugated β-actin mouse monoclonal antibody, HRP-conjugated AffiniPure goat anti-mouse or goat anti-rabbit IgG (H + L), fluorescein (FITC)-conjugated AffiniPure goat anti-rabbit IgG (H + L), and Cy3-conjugated AffiniPure goat anti-rabbit IgG (H + L) were purchased from Proteintech. Cell culture reagents were purchased from Gibco.

Quercetin (purity ≥ 95%), TBHP and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO, United States). 3-Methyladenine (3-MA) and Selisistat (EX-527) were purchased from Selleck Chemical (Houston, TX, United States). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Primary antibodies for MMP13 was purchased from Abcam (Cambridge, United Kingdom). Antibody for LC3, p62, cleaved-caspase3 and SIRT1 were purchased from Cell Signaling Technologies (Danvers, MA, United States) and antibody for Aggrecan was purchased from Millipore (Burlington, MA, United States). Horseradish peroxidase (HRP)-conjugated β-actin mouse monoclonal antibody, HRP-conjugated affinipure goat anti-mouse or goat anti-rabbit IgG (H + L), fluorescein (FITC)-conjugated affinipure goat anti-mouse or goat anti-rabbit IgG (H + L) and Cy3-conjugated affinipure goat anti-mouse or goat anti-rabbit IgG (H + L) were purchased from Proteintech (Wuhan, China).