Changes in Bcl-2 phosphorylation in HeLa cells were assessed using the Muse™ Bcl-2 Activation Dual Detection Assay kit (Merck-Millipore, Guyancourt, France) according to the manufacturer’s instructions. Two direct conjugated antibodies were used in the kit, i.e., phospho-specific anti-phospho-Bcl-2 (Ser70)-Alexa Fluor® 555 and a conjugated anti-Bcl-2-PECy5 antibody to measure total Bcl-2 expression levels. The degree of activation of the Bcl-2 pathway was assessed by measuring Bcl-2 phosphorylation relative to total Bcl-2 expression in the tested cells.
Muse bcl 2 activation dual detection assay kit
Manufactured by Merck Group
Sourced in France
The Muse™ Bcl-2 Activation Dual Detection Assay kit is a flow cytometry-based assay designed to detect and quantify the activation of the Bcl-2 protein, a key regulator of apoptosis (programmed cell death). The kit provides a simple and reliable method to measure the levels of active and inactive forms of Bcl-2 in cell samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using muse bcl 2 activation dual detection assay kit
1
Bcl-2 Phosphorylation Analysis in HeLa Cells
2
Bcl-2 Phosphorylation Profiling in HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Changes in Bcl-2 phosphorylation in HeLa cells (density of cells = 3 × 105/mL) were assessed using the Muse™Bcl-2 Activation Dual Detection Assay Kit (Merck-Millipore, Guyancourt, France) according to the manufacturer’s instructions. Two direct conjugated antibodies were used in the kit, i.e., phospho-specific anti-phospho-Bcl-2 (Ser70)-Alexa Fluor® 555 and a conjugated anti-Bcl-2-PECy5 antibody to measure total Bcl-2 expression levels. Evaluation of the degree of activation of the Bcl-2 pathway was performed by measuring the phosphorylation of Bcl-2 relative to the total expression of Bcl-2 in the tested cells. The experiment was repeated 3 times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!