TRIM1-TRIM32 and TRIM37 were obtained from Dr. Andrea Ballabio(Texaschildren’s hospital)53 (link); TRIM35, 39, 41, 43, 44, 46, 47, 52, 58, and 62 were from Dr. Walther Mothes (Yale University)54 (link); and TRIM36, 48, 49, 56 59, 60, 61, 63, and 72 were from Open Biosystems and Invitrogen. Expression plasmid for RIG-I-2CARD and TRIM25 was described previously27 (link) and TRIM34 was obtained from Dr. Paul D. Bieniasz(Howard Hughes Medical Institute)55 (link)., HA-NIK (Addgene plasmid 27554) was from Dr. Shao-Cong Sun(MD Anderson Cancer Center) 12. β-TrCP and NF-κB2 (p100) were received from Dr. Michele Pagano (New York University)56 (link). TRIM9 and β -TrCP mutants were generated using a PCR site-directed mutagenesis kit (Stratagene) and subcloned into pCDH vector (System Biosciences) or pEBG vector. Lentivirus-shRNAs against TRIM9 were purchased from OpenBiosystem. The plasmid pRL-TK constitutively expressing Renilla luciferase was purchased from Promega. IKKα, IKK β, p65, p50, IκBα were gifted from Dr. EbrahimZandi (University of Southern California)57 (link). Firefly luciferase reporter plasmids under the ISRE (ISG54), NF-AT, AP-1 or NF-κB promoters were purchased from Stratagene. TRAF6, TAB2, IKKε, TBK1, and ubiquitin-expressing plasmids were described previously29 (link).
Prl tk constitutively expressing renilla luciferase
Manufactured by Promega
The PRL-TK plasmid constitutively expresses Renilla luciferase, a bioluminescent reporter enzyme. The plasmid contains the thymidine kinase promoter from Herpes simplex virus, which drives continuous expression of the Renilla luciferase gene.
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2 protocols using prl tk constitutively expressing renilla luciferase
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Plasmid Sources for TRIM and Signaling Proteins
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Plasmid Sources for TRIM and Signaling Proteins
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TRIM1-TRIM32 and TRIM37 were obtained from Dr. Andrea Ballabio(Texaschildren’s hospital)53 (link); TRIM35, 39, 41, 43, 44, 46, 47, 52, 58, and 62 were from Dr. Walther Mothes (Yale University)54 (link); and TRIM36, 48, 49, 56 59, 60, 61, 63, and 72 were from Open Biosystems and Invitrogen. Expression plasmid for RIG-I-2CARD and TRIM25 was described previously27 (link) and TRIM34 was obtained from Dr. Paul D. Bieniasz(Howard Hughes Medical Institute)55 (link)., HA-NIK (Addgene plasmid 27554) was from Dr. Shao-Cong Sun(MD Anderson Cancer Center) 12. β-TrCP and NF-κB2 (p100) were received from Dr. Michele Pagano (New York University)56 (link). TRIM9 and β -TrCP mutants were generated using a PCR site-directed mutagenesis kit (Stratagene) and subcloned into pCDH vector (System Biosciences) or pEBG vector. Lentivirus-shRNAs against TRIM9 were purchased from OpenBiosystem. The plasmid pRL-TK constitutively expressing Renilla luciferase was purchased from Promega. IKKα, IKK β, p65, p50, IκBα were gifted from Dr. EbrahimZandi (University of Southern California)57 (link). Firefly luciferase reporter plasmids under the ISRE (ISG54), NF-AT, AP-1 or NF-κB promoters were purchased from Stratagene. TRAF6, TAB2, IKKε, TBK1, and ubiquitin-expressing plasmids were described previously29 (link).
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