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Dionex ionpac as19 4 μm

Manufactured by Thermo Fisher Scientific

The Dionex IonPac AS19-4 μm is an analytical column designed for the separation and determination of anions. It features a particle size of 4 μm and is intended for use in ion chromatography applications.

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2 protocols using dionex ionpac as19 4 μm

1

Comprehensive Ionic Chromatography-HRMS Analysis

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The instrumental analyses were performed by a Dionex ICS-5000+ Ionic Chromatography (IC) system (Sunnyvale, CA, USA) coupled to a Thermo Q-Exactive Orbitrap™ (Thermo Scientific, San Jose, CA, USA), equipped with a heated electrospray ionization (HESI) source. For the analyte separation, a Thermo Scientific Dionex IonPac AS19-4 μm (2 × 250 mm, 4 μm particle size) with a Dionex IonPac AG19-4 μm guard column (2 × 50 mm) was used. The gradient, all IC, and HRMS parameters were described in Chiesa et al. [7 (link)].
ChromeleonTM (Thermo Fisher Scientific, San Jose, CA, USA) and XcaliburTM 3.0 (Thermo Fisher Scientific, Waltham, MA) software was used to control the IC and HRMS system, respectively.
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2

Ion Chromatography Analysis of Samples

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The analyses were accomplished by an Ionic Chromatography (IC) Dionex ICS-5000+ system (Sunnyvale, CA, USA) made up of a Dual Pump (DP), a Conductivity Detector (EG), a Detector/Chromatography Module (DC) and an Autosampler (AS-AP). The ion chromatography separation column was a Thermo Scientific Dionex IonPac AS19-4 μm (2 × 250 mm, 4 μm particle size) with a guard column Dionex IonPac AG19-4 μm (2 × 50 mm) maintained at 30 °C. The eluent flow rate was 0.30 mL/min with a gradient from 15 mM KOH (aq), held for 8 min, increased to 55 mM KOH (aq) at 20 min, held in these conditions for 4 min and back to 15 mM KOH (aq) at 24.1 min, with a cycle time of 30 min. The KOH eluent was neutralized using Dionex anion self-regenerating suppressor set to 50 mA (ASRS II, 4 mm). Chromeleon TM software (Thermo Fisher Scientific, Waltham, MA) was used to control the IC system and to elaborate the data obtained.
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