3 6 dioxa 1 8 octanedithiol dodt

3,6-dioxa-1,8-octanedithiol (DODT) was purchased from Sigma-Aldrich (Spain). Solvents and reagents for peptide synthesis (N,N-dimethylformamide (DMF), N,N-diisopropylethylamine (DIEA), dichloromethane (DCM), trifluoroacetic acid (TFA), N,N-diisopropylcarbodiimide (DIPCI), and triisopropylsilane (TIS)), as well as HPLC-grade acetonitrile (CAN) were purchased from Carlo Erba-SDS (Sabadell, Spain). Fmoc-protected amino acids, Fmoc-Rink amide (MBHA) resin, N-hydroxybenzotriazole (HOBt), and 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Iris Biotech (Marktredwitz, Germany).
Dulbecco’s modified Eagle medium (DMEM), trypsin-EDTA, fetal bovine serum (FBS), l-glutamine, and penicillin-streptomycin antibiotic solution (pen/strep solution) were purchased from Gibco/Thermo Fischer (Waltham, MA, USA). Eagle’s minimum essential medium (EMEM) was purchased from Sigma-Aldrich (Madrid, Spain). CellTiter-Blue^® cell viability reagent was purchased from Promega (Madrid, Spain). CellEvent^TM ReadyProbe^®, CellRox^® Deep Red reagent, and Live/Dead^TM viability/cytotoxicity kit were purchased from Invitrogen/Thermo Fisher (Waltham, MA, USA).

Fmoc-protected amino acids and synthesis resin, 2-Chlorotrityl chloride were purchased from Akaal Organics (Long Beach, CA). Dimethylformamide (DMF), N-methyl pyrrolidone (NMP), Acetonitrile and Methyl-tert. Butyl Ether (MTBE) were purchased from Fisher Bioreagents (Fair Lawn, NJ). 2-(6-Chloro-1-H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU) was purchased from AAPPTEC (Louisville, KY). Piperidine and Dichloromethane (DCM) were from EMD-Millipore (Billerica, MA). Diisopropylethylamine (DIEA), N-Methyl-morpholine (NMM), Triisoprpopyl-silane, 3,6-dioxa-1,8-octanedithiol (DODT) and trifluoroacetic acid (TFA) were purchased from Sigma–Aldrich.