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Slug sirna

Manufactured by Thermo Fisher Scientific
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SLUG siRNA is a laboratory tool used to temporarily reduce the expression of the SLUG gene in cell cultures. It is a sequence-specific, double-stranded RNA molecule designed to target and degrade the SLUG mRNA, thereby suppressing the production of the SLUG protein.

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Lab products found in correlation

Smartpool, by Thermo Fisher Scientific (1 mentions) On targetplus non targeting pool sirna, by Thermo Fisher Scientific (1 mentions) Dharmafect solution, by Thermo Fisher Scientific (1 mentions) Oligofectamine, by Thermo Fisher Scientific (1 mentions)

2 protocols using slug sirna

1

Modulating FILIP1L and SLUG in Ovarian Cancer

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ON-TARGETplus Non-Targeting siRNA Pool (D-001810-10), FILIP1L siRNA (#7 (J-019458-07) and SMARTpool (set of 4 sequences; L-019458-00)) and SLUG siRNA (#8 (J-017386-08) and SMARTpool (L-017386-00)) were purchased from Thermo Scientific. HEY and OVCAR8 cells were transfected with equimolar amounts of either non-Targeting or FILIP1L and/or SLUG siRNA using Dharmafect solution following the manufacturer's protocols (Thermo Scientific). After a 48 h transfection, the cell lysates were subjected to qRT-PCR and immunoblot analysis.
Kwon M., Kim J.H., Rybak Y., Luna A., Choi C.H., Chung J.Y., Hewitt S.M., Adem A., Tubridy E., Lin J, & Libutti S.K. (2016). Reduced expression of FILIP1L, a novel WNT pathway inhibitor, is associated with poor survival, progression and chemoresistance in ovarian cancer. Oncotarget, 7(47), 77052-77070.
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2

Silencing HMGA2 and Luciferase in MCF7 Cells

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RNA against HMGA2 SMARTpool siRNA (CCAGGAAGCAGCAGCAAGA) and firefly luciferase (CGTACGCGGAATACTTCGA) were synthesized by Dharmacon (Lafayette, CO, USA). MCF7/PAR1-N18 cells were transfected overnight with 0.2 μM siRNA using Oligofectamine (Invitrogen) in serum-free RPMI 1640 without antibiotics. Slug siRNA and ZEB1 siRNA were purchased premade from Thermo Scientific (Tewksbury, MA, USA). Cells were recovered for 24 h in 10% fetal bovine serum before use in assays.
Yang E., Cisowski J., Nguyen N., O’Callaghan K., Xu J., Agarwal A., Kuliopulos A, & Covic L. (2015). Dysregulated protease activated receptor 1 (PAR1) promotes metastatic phenotype in breast cancer through HMGA2. Oncogene, 35(12), 1529-1540.
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