Hearts were dissected free from the surrounding connective tissue, and fixed with 4% paraformaldehyde, embedded in paraffin, and then cut into slices using a microtome (Leica RM 2235 or Leica CM1850UV; Leica, Solms, Germany). The slices were then mounted onto glass slides, and histological examinations were performed. Immunohistochemistry was performed using Histofine Simple Stain kit (Nichirei, Tokyo, Japan), according to the manufacturer’s instructions. Briefly, sections were deparaffinised with xylene and then rehydrated in a descending ethanol series. Sections were treated with 3% H2O2 in methanol for 15 min to inactivate endogenous peroxidases and then incubated with a primary antibody against p62 (rabbit anti-p62 antibody, 1:200; Proteintech); LC3 (rabbit anti-LC3 antibody, 1:200; Proteintech); CD68 (rabbit anti-CD68 antibody, 1:250; Abcam) at room temperature for 1 h. All sections were examined under an Olympus BX40 upright light microscope (Olympus, Tokyo, Japan).
Leica rm2235
Manufactured by Leica camera
Sourced in Germany, China, United States
The Leica RM2235 is a microtome designed for sectioning paraffin-embedded tissue samples. It features a full-stroke length of 70 mm and a section thickness range of 0.5 to 100 μm. The instrument is motorized and allows for precision cutting of specimens for histological analysis.
Automatically generated - may contain errors
Lab products found in correlation
Transmission electron microscope, by Hitachi (2 mentions)
Ds ri2 camera, by Olympus (2 mentions)
Dp72 microscope, by Olympus (2 mentions)
Silicon coated glass slides, by Leica Biosystems (2 mentions)
Hematoxylin, by Merck Group (2 mentions)
Rabbit anti cd68 antibody, by Abcam (1 mentions)
Bx40 upright light microscope, by Olympus (1 mentions)
Histofine simple stain kit, by Nichirei Biosciences (1 mentions)
Rabbit anti lc3 antibody, by Proteintech (1 mentions)
Leica cm1850uv, by Leica camera (1 mentions)
Rabbit anti p62 antibody, by Proteintech (1 mentions)
Vs200, by Olympus (1 mentions)
Eclipse te2000 e microscope, by Nikon (1 mentions)
Roti histofix, by Carl Roth (1 mentions)
Histocore arcadia, by Leica camera (1 mentions)
Eclipse e200, by Nikon (1 mentions)
Surgipath x tra adhesive precleaned microslides, by Leica Biosystems (1 mentions)
Leica dmlb, by Leica camera (1 mentions)
Leica dfc290, by Leica camera (1 mentions)
Dm3000, by Leica camera (1 mentions)
31 protocols using leica rm2235
1
Histological Analysis of Heart Tissue
2
Histological Evaluation of Cardiac Tissue
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In short, after perfusion, the left ventricular tissues were fixed (4% PFA), dehydrated (graded alcohols), cleared (xylene), paraffin-infiltrated and embedded, in sequence. The microtome (Leica RM2235, Leica microscopic system Shanghai Co., Ltd., China) was used to slice the tissues. The sections were dewaxed (xylene), hydrated (alcohol), stained with hematoxylin (5 minutes), differentiated with 1% hydrochloric acid alcohol (2 seconds), stained with eosin (8 minutes), dehydrated with alcohol and cleared in xylene. Finally, the mounted slides were photographed using a digital slice scanner (Olympus VS200, Olympus Corporation, Japan).
3
Histochemical Analysis of Transgenic Plants
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Shoot tips and series of internodes of stem from PtrHB4pro: GUS transgenic plants were hand‐sectioned and then incubated in 50% acetone (v/v) for 10 min on ice, and incubated in GUS stain solution (100 mm sodium phosphate (pH7.0), 10 mm EDTA, 0.5 mm ferricyanide, 0.5 mm ferrocyanide, 0.1% Triton X‐100, 20% methanol and 2 mm X‐Gluc) at 37 °C for 2 h. Following staining, sections were cleared by 75% ethanol and photographed. Shoot tips and stem segments with 3 mm length at different internodes from transgenic and wild type plants were fixed in formaldehyde‐acetic acid solution (formaldehyde:glacial acetic acid:ethanol [1 : 1 : 18]) for 24 h, dehydrated in graded ethanol series, and embedded into paraplast. The samples were sectioned to 10 μm thick using rotary microtome of Leica RM2235 (Leica, http://www.leica-microsystems.com/products ). The sections were stained with toluidine blue and observed under a light microscope of OLYMPUS BX51 (OLYMPUS). For lignin staining, sections were immersed in 1% phloroglucinol (w/v) in 12% HCl for 5 min and immediately observed with a light microscope.
4
Ultrastructural Analysis of Mitochondria in Transplanted Liver
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The transplanted liver was double-fixed by glutaraldehyde and osmic acid, dehydrated by gradient acetone, immersed in embedding medium, ultrathin sectioned using an automatic microtome (LeicaRM2235, Leica, Germany), and stained with 1% uranyl acetate. The tissue slices were observed and filmed under a transmission electron microscope (Hitachi, Japan) to observe the status of mitochondria in the hepatocytes.
5
Paraffin-Embedded Tumor Sample Histology
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Transgenic tumor samples were fixated with Roti®-Histofix (Roth) and embedded in paraffin. Paraffin blocks were sliced at 4 µm thickness using LEICA RM2235 (Leica) and transferred to a specimen slide. Staining was performed for hematoxylin and eosin as well as Phox2b. Images were acquired using a Nikon Eclipse TE2000-E microscope with a 20 × objective and 1.5 manual magnification.
6
Testes Histology with H&E Staining
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Testes were fixed in Bouin’s solution. After dehydration, tissues were embedded with paraffin (HistoCore Arcadia, Leica, Mannheim, Germany). Paraffin-embedded tissues were then cut for 5 µm by microtome (Leica RM2235, Leica, Mannheim, Germany). After rehydration, sections were stained with hematoxylin and eosin (H&E). Images were examined by using a microscope (ECLIPSE E200, Nikon, Tokyo, Japan), and captured by CCD (MS60, MshOt, Guangzhou, China).
7
Ocular Globe Histological Preparation
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The inclusion criteria were mainly based on the preserved quality of the globes. One globe was used from each species and was dissected to examine the cornea and limbus.
The tissue was fixed in 10% neutral buffered formalin at room temperature (RT) overnight. The tissue was then placed in a tissue-embedding cassette and processed. Each processed ocular globe was then embedded in paraffin wax using a mold. A microtome (Leica RM2235; Leica, Germany) was used to make 4 µm sections that were mounted on slides (Surgipath X-tra Adhesive Precleaned Microslides; Leica Biosystems, USA). Twenty slides were made from each embedded block. All slides were placed in an incubator (Leec, UK) at 37°C overnight.
The tissue was fixed in 10% neutral buffered formalin at room temperature (RT) overnight. The tissue was then placed in a tissue-embedding cassette and processed. Each processed ocular globe was then embedded in paraffin wax using a mold. A microtome (Leica RM2235; Leica, Germany) was used to make 4 µm sections that were mounted on slides (Surgipath X-tra Adhesive Precleaned Microslides; Leica Biosystems, USA). Twenty slides were made from each embedded block. All slides were placed in an incubator (Leec, UK) at 37°C overnight.
8
Meat Composition and Fiber Analysis
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AOAC standard procedures were used to evaluate the content of crude protein (CP), moisture (MSTR), total ash (Ash), and ether extract (EE). The MSTR content of the meat samples was determined by drying the meat samples in an oven at 105 °C. The CP and EE contents were estimated using the Soxhlet extraction method and the Kjeldahl technique, respectively. The meat samples were burned in a crucible at a temperature of 550 °C for 4 h to determine the content of ash.
The traditional hematoxylin and eosin (H&E) staining method was used to measure the total number, total area, density, and diameter of the muscle fibers. Slides were imaged via a Leica RM2235 slide scanner (Leica RM2235, Nussloch, Germany), and images were acquired using CaseViewer 6.0 software (3DHistech, Ltd., Budapest, Hungary).
The traditional hematoxylin and eosin (H&E) staining method was used to measure the total number, total area, density, and diameter of the muscle fibers. Slides were imaged via a Leica RM2235 slide scanner (Leica RM2235, Nussloch, Germany), and images were acquired using CaseViewer 6.0 software (3DHistech, Ltd., Budapest, Hungary).
9
Root Canal Histological Evaluation
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Each canal was irrigated and filled overnight with 1 mL of 10% buffered formalin to allow for fixation of any remaining biofilm, as described earlier [14 (link)]. Roots were embedded in paraffin.
The roots were divided into three regions as—apical region (with sub-regions at 1, 2, and 3 mm) and middle region (with sub-regions at 4, 5, and 6 mm). Four slices at 1 mm increments were obtained at each sub-region using a rotary microtome (Leica RM2235, Leica, Wetzlar, Germany) for a total of 24 sections per root. 12 sections were stained using hematoxylin and eosin (H&E) and 12 sections were stained using modified Brown and Brenn (B&B). The H&E stained and B&B stained cross-sections were further examined using an optical stereomicroscope (Leica DMLB, Leica, Wetzlar, Germany) and images were acquired using a camera (Leica DFC290, Leica, Wetzlar, Germany). For further analysis, B&B stained slides were examined at 13.5× magnification. Resulting images were subjected to morphometric analysis [21 (link)].
The roots were divided into three regions as—apical region (with sub-regions at 1, 2, and 3 mm) and middle region (with sub-regions at 4, 5, and 6 mm). Four slices at 1 mm increments were obtained at each sub-region using a rotary microtome (Leica RM2235, Leica, Wetzlar, Germany) for a total of 24 sections per root. 12 sections were stained using hematoxylin and eosin (H&E) and 12 sections were stained using modified Brown and Brenn (B&B). The H&E stained and B&B stained cross-sections were further examined using an optical stereomicroscope (Leica DMLB, Leica, Wetzlar, Germany) and images were acquired using a camera (Leica DFC290, Leica, Wetzlar, Germany). For further analysis, B&B stained slides were examined at 13.5× magnification. Resulting images were subjected to morphometric analysis [21 (link)].
10
Adipocyte Diameter Quantification
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The paraformaldehyde-fixed backfat samples were embedded in paraffin. The serial tissue sections were cut using cryostat (Leica RM2235; Leica company, Wetzlar, Germany) and were then stained with hematoxylin/eosin. The sections were viewed at 200× magnification using microscope (Leica DM3000, Germany), and five areas were randomly selected in each sample for measuring the diameter of adipocyte.
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