Primary human umbilical vein vascular endothelium cells (HUVEC, ATCC® PCS‐100‐010™) were obtained from ATCC and maintained in endothelial cell growth medium including growth supplements (EGM, CC‐3124, Lonza). The MCF‐7 luminal‐like breast cancer cell line was obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China) and maintained in Dulbecco's Modified Eagle's Medium (DMEM) with high glucose supplemented with 100 U/mL of penicillin, 100 μg/mL streptomycin and 10% foetal bovine serum (FBS). The cells were incubated at 37°C in a humidified incubator supplemented with 5% CO2. All the siRNAs were transfected into HUVECs using Lipofectamine 3000 (Invitrogen) at a final concentration of 100 nmol/L.
Atcc pcs 100 010
Manufactured by American Type Culture Collection
Sourced in United States
The ATCC® PCS‐100‐010™ is a laboratory equipment product designed for cell culture applications. It serves as a tool for maintaining and propagating cells in vitro. The product provides a controlled environment for cell growth and experimentation, without further interpretation of its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Vegf165, by Thermo Fisher Scientific (1 mentions)
Egm 2 bulletkit, by Lonza (1 mentions)
Hb 8065, by American Type Culture Collection (1 mentions)
Myricetin, by Merck Group (1 mentions)
Huvec cell line, by American Type Culture Collection (1 mentions)
Rpim1640, by Thermo Fisher Scientific (1 mentions)
Pcs 100 030, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Huvecs, by ScienCell (1 mentions)
Dulbecco modified eagle medium (dmem), by ScienCell (1 mentions)
8 protocols using atcc pcs 100 010
1
Culturing Human Endothelial and Breast Cancer Cells
2
Endothelial Differentiation of Adipose Stem Cells
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The differentiation process started at 50–60% confluency of AMSCs. The AMSC culture was stimulated with endothelial growth medium (EGM) composed of Endothelial Basal Medium-2 (Gibco, Grand Island, NY, USA), growth supplements (containing hydrocortisone, human fibroblast growth factor (hFGF-b), R3-insulin-like growth factor-1 (R3-IGF-1), ascorbic acid, human epithelial growth factor (hEGF), GA-1000, heparin), 2% FBS (EGM-2 Bullet Kit; Lonza, Walkersville, MD, USA), and 50 ng/ml VEGF-165 (Peprotech, Rocky Hill, NJ, USA). The cells were maintained at 37 °C with 5% CO2/95% air and 90% relative humidity, and the medium was changed every 3 days. The cells were detached from the monolayer with 0.25% Trypsin–EDTA, and collected for analysis after 10 days of stimulation for EC differentiation.
Human umbilical vein endothelial cells (HUVECs) (ATCC® PCS-100-010™; ATCC) were used as a positive control.
Human umbilical vein endothelial cells (HUVECs) (ATCC® PCS-100-010™; ATCC) were used as a positive control.
3
Cell Line Culture Protocols
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The TM4 cell line was purchased from iCell Bioscience, Inc. (Shanghai, China). The HepG2 [American Type Culture Collection (ATCC)® HB-8065™] and human umbilical vein endothelial cells (HUVECs; ATCC® PCS-100-010™) cell lines were purchased from ATCC (Manassas, VA, USA). TM4 cells and HUVECs were cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in 5% CO2. HepG2 cells were cultured in DMEM supplemented with 10% FBS at 37 °C in 5% CO2.
4
Overexpressing miR-200b-3p in HUVECs
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Human umbilical vein endothelial cells (HUVECs) were purchase from ATCC(ATCC®PCS-100-010). Cultures of HUVECs were grown on fibronectin-coated plates; maintained in EGM (Lonza) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. micrONTMhsa-miR-200b-3p mimic (50 nM) or negative controls (micrON™ miRNA mimic NC #22, RIOBOBIO, Guangzhou, China) were transfected using Lipofectamine 3000 according to manufacturer’s protocol (Invitrogen). Further analysis was performed after 48 h of transfection.
5
HUVEC Cell Line Myricetin Treatment
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HUVEC cell line was purchased from American Type Culture Collection (ATCC® PCS-100-010). Myricetin was commercially obtained from Sigma-Aldrich, USA (Catalog No: M6760). All cell culture chemicals were bought either from Sigma-Aldrich or Merck. Antibody eNOS and β-actin mouse mAb were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All the other chemicals were bought either from Sigma-Aldrich, Merck or other standard suppliers.
6
Culturing and Maintaining Breast Cancer and Endothelial Cells
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Primary human umbilical vein vascular endothelium cells (HUVECs, ATCC® PCS-100-010™) were purchased from American Type Culture Collection (ATCC, Manassas, USA) and maintained in endothelial cell growth medium including growth supplements (EGM, CC-3124, Lonza, Walkersville, MD, USA). 4T1 mouse breast cancer cell line and MDA-MB-231 human breast cancer cell line were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). The 4T1 cells were maintained in RPIM1640 (Gibco, Carlsbad, CA, USA), and MDA-MB-231 cells were cultured in L-15 (Gibco). Both RPIM1640 and L-15 media contain 10% fetal bovine serum (FBS; Gibco), 100 U/mL penicillin, and 100 μg/mL phytomycin. HUVECs and 4T1 cells were incubated at 37 °C with 5% CO2, and MDA-MB-231 cells were incubated at 37 °C without CO2.
7
Culturing Primary Human Umbilical Vein Endothelial Cells
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Primary human umbilical vein endothelial cells (HUVECs; ATCC PCS-100-010; American Type Culture Collection; Manassas, VA, USA) were grown in an incubator with vascular cell basal medium (ATCC PCS-100-030) and endothelial cell growth kit-BBE (ATCC PCS-1-040) and 100 U/ml penicillin-streptomycin (Sigma-Aldrich, USA) under humidified atmosphere of 95% air and 5% CO2 at 37°C.[8 (link)]
8
Regulation of Pulmonary Cell Signaling
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Human pulmonary arterial smooth muscle cell (H‐PASMC; ATCC® PCS‐100‐023™) and human umbilical vein endothelial cells (HUVECs; ATCC® PCS‐100‐010™) were purchased from American Type Culture Collection (ATCC). H‐PASMC and HUVECs were cultured in Dulbecco's modified Eagle medium (4.5 g/L D‐glucose; DMEM, ScienCell), both supplemented with 10% foetal bovine serum (FBS, Gibco) and 100 units/mL penicillin/streptomycin in a humidified incubator at 37°C with 5% CO2. The miR‐508‐3p mimics, miR‐508‐3p inhibitor, normal control (NC) oligonucleotides and the small‐interfering RNA (siRNA) targeting NR4A3 mRNA kit were purchased from GenePharma. Cell transfection was performed with Lipofectamine™ 2000 transfection reagent (Invitrogen) according to the manufacturer's instructions.
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