IAA was quantified by liquid chromatography-mass spectrometry (LC-MS), essentially as described [55 (link)]. Briefly, 50 mg of frozen tissue was homogenized in 500 μL of extraction buffer (isopropanol: double distilled water: acetic acid = 2:1:0.002, vol/vol/vol). After shaking on ice for 30 min, 1 mL dichloromethane was added to each sample and shaken for another 30 min. Then, samples were centrifuged at 13,000 g for 5 min at 4 °C; the lower phase was dried under a stream of nitrogen gas, and redissolved with methyl alcohol. Each sample solution was injected into the reverse-phase C18 HPLC column (2.1 mm × 100 mm, 2.7 μm, Agilent, USA) for LC-MS (Shimadzu LC-30 ultrahigh performance liquid chromatography system, Shimadzu, Japan; AB Sciex QTRAP 5500, SCIEX, USA) analysis. An IAA standard (≥98%, 45,533, Sigma, USA) was used as the external standard.
Lc 30 ultrahigh performance liquid chromatography system
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu LC-30 is an ultrahigh performance liquid chromatography (UHPLC) system designed for high-resolution separation and analysis of complex samples. It features a low-volume flow path and high-speed data acquisition to enable efficient chromatographic separations.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using lc 30 ultrahigh performance liquid chromatography system
1
Quantification of Indole-3-Acetic Acid by LC-MS
2
Quantifying IAA via LC-MS
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IAA was quanti ed by liquid chromatography-mass spectrometry (LC-MS), essentially as described [44] . Brie y, 50 mg of frozen tissue was homogenized in 500 μL of extraction buffer (isopropanol: double distilled water: acetic acid= 2:1:0.002, vol/vol/vol). After shaking on ice for 30 min, 1 mL dichloromethane was added to each sample and shaken for another 30 min. Then, samples were centrifuged at 13,000 g for 5 min at 4 °C; the lower phase was dried under a stream of nitrogen gas, and redissolved with methyl alcohol. Each sample solution was injected into the reverse-phase C 18 HPLC column (2.1mm × 100 mm, 2.7 μm, Agilent, USA) for LC-MS (Shimadzu LC-30 ultrahigh performance liquid chromatography system, Shimadzu, Japan; AB Sciex QTRAP 5500, SCIEX, USA) analysis. An IAA standard (≥98%, 45533, Sigma, USA) was used as the external standard.
3
Quantification of IAA by LC-MS
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IAA was quanti ed by liquid chromatography-mass spectrometry (LC-MS), essentially as described [41] . Brie y, 50 mg of frozen tissue was homogenized in 500 µL of extraction buffer (isopropanol: double distilled water: acetic acid = 2:1:0.002, vol/vol/vol). After shaking on ice for 30 min, 1 mL dichloromethane was added to each sample and shaken for another 30 min. Then, samples were centrifuged at 13,000 g for 5 min at 4 °C; the lower phase was dried under a stream of nitrogen gas, and redissolved with methyl alcohol. Each sample solution was injected into the reverse-phase C 18 HPLC column (2.1 mm × 100 mm, 2.7 µm, Agilent, USA) for LC-MS (Shimadzu LC-30 ultrahigh performance liquid chromatography system, Shimadzu, Japan; AB Sciex QTRAP 5500, SCIEX, USA) analysis. An IAA standard (≥ 98%, 45533, Sigma, USA) was used as the external standard.
4
Quantifying IAA levels via LC-MS
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IAA was quanti ed by liquid chromatography-mass spectrometry (LC-MS), essentially as described [55] . Brie y, 50 mg of frozen tissue was homogenized in 500 μL of extraction buffer (isopropanol: double distilled water: acetic acid= 2:1:0.002, vol/vol/vol). After shaking on ice for 30 min, 1 mL dichloromethane was added to each sample and shaken for another 30 min. Then, samples were centrifuged at 13,000 g for 5 min at 4 °C; the lower phase was dried under a stream of nitrogen gas, and redissolved with methyl alcohol. Each sample solution was injected into the reverse-phase C 18 HPLC column (2.1mm × 100 mm, 2.7 μm, Agilent, USA) for LC-MS (Shimadzu LC-30 ultrahigh performance liquid chromatography system, Shimadzu, Japan; AB Sciex QTRAP 5500, SCIEX, USA) analysis. An IAA standard (≥98%, 45533, Sigma, USA) was used as the external standard.
5
Quantifying IAA levels via LC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IAA was quanti ed by liquid chromatography-mass spectrometry (LC-MS), essentially as described [55] . Brie y, 50 mg of frozen tissue was homogenized in 500 μL of extraction buffer (isopropanol: double distilled water: acetic acid= 2:1:0.002, vol/vol/vol). After shaking on ice for 30 min, 1 mL dichloromethane was added to each sample and shaken for another 30 min. Then, samples were centrifuged at 13,000 g for 5 min at 4 °C; the lower phase was dried under a stream of nitrogen gas, and redissolved with methyl alcohol. Each sample solution was injected into the reverse-phase C 18 HPLC column (2.1mm × 100 mm, 2.7 μm, Agilent, USA) for LC-MS (Shimadzu LC-30 ultrahigh performance liquid chromatography system, Shimadzu, Japan; AB Sciex QTRAP 5500, SCIEX, USA) analysis. An IAA standard (≥98%, 45533, Sigma, USA) was used as the external standard.
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