Western blot experiments was performed as previously described24 (link). Briefly, protein was extracted with RIPA buffer (Byotime) supplemented with protease inhibitor cocktail (Roche). Total protein concentration was quantified with BCA Protein Assay Kit (Thermo). An equal amount of total proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane (Millipore). Then block the membrane with 5% non-fat milk, incubated with primary antibodies at 4 °C overnight and followed by secondary antibody for 1 h. The primary antibodies used were as follows: anti-ACSS1 (Invitrogen, PA5-59392), anti-ACSS2 (Cell Signaling Technology, 3568 S), anti-ACSS3 (Invitrogen, PA5-80305), anti-Tubulin (Proteintech, 66031–1), anti-acetyl-histone H4 (Millipore, 06–598), anti-histone H3 (CST, 4499), anti-acetyl-histone H3 (Millipore, 06–599), and anti-histone H4 (CST, 13919).
Anti acss2
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-ACSS2 is a primary antibody that specifically recognizes the ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2) protein. ACSS2 is an enzyme involved in the metabolism of acetate to acetyl-CoA, which is a key metabolic intermediate. The anti-ACSS2 antibody can be used to detect and study the expression and localization of the ACSS2 protein in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti acetyl histone h4, by Merck Group (3 mentions)
Anti acetyl histone h3, by Merck Group (3 mentions)
Polyvinylidene difluoride membrane, by Merck Group (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
Anti acss1, by Thermo Fisher Scientific (2 mentions)
Anti acss3, by Thermo Fisher Scientific (2 mentions)
Anti tubulin, by Proteintech (2 mentions)
Puromycin, by Merck Group (1 mentions)
D luciferin potassium salt, by PerkinElmer (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Nupage gel, by Thermo Fisher Scientific (1 mentions)
Anti β tubulin, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Protein assay, by Bio-Rad (1 mentions)
Irdye 680lt, by LI COR (1 mentions)
Anti histone h3, by Active Motif (1 mentions)
4 protocols using anti acss2
1
Western Blot for Protein Analysis
2
Comprehensive Cell Signaling Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-actin (Santa Cruz Biotechnology; Dallas, TX, United States), Anti-ACSS2, Anti-FasN,Anti-beta Tubulin (Cell Signaling Technology; Danvers, MA, United States). Puromycin, Polybrene crystal violet (Sigma Aldrich, St. Louis, MO, United States), D-luciferin potassium salt (Perkin Elmer, Waltham, MA, United States).
3
Western Blot for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot experiments was performed as previously described24 (link). Briefly, protein was extracted with RIPA buffer (Byotime) supplemented with protease inhibitor cocktail (Roche). Total protein concentration was quantified with BCA Protein Assay Kit (Thermo). An equal amount of total proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane (Millipore). Then block the membrane with 5% non-fat milk, incubated with primary antibodies at 4 °C overnight and followed by secondary antibody for 1 h. The primary antibodies used were as follows: anti-ACSS1 (Invitrogen, PA5-59392), anti-ACSS2 (Cell Signaling Technology, 3568 S), anti-ACSS3 (Invitrogen, PA5-80305), anti-Tubulin (Proteintech, 66031–1), anti-acetyl-histone H4 (Millipore, 06–598), anti-histone H3 (CST, 4499), anti-acetyl-histone H3 (Millipore, 06–599), and anti-histone H4 (CST, 13919).
4
Western Blot Analysis of Acetylation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein lysates were prepared in radioimmunoprecipitation assay (RIPA) buffer (Pierce) with protease inhibitor cocktail (Sigma), and total protein concentration was determined by protein assay (Bio-Rad). Proteins were separated using precast NuPAGE gels (Invitrogen, Life Technologies) and transferred onto a nitrocellulose membrane. Protein detection and quantification were done with a LI-COR Odyssey infrared flatbed scanner (LI-COR Biosciences). The primary antibodies used were as follows: anti-ACSS1, 1:1,000 (Sigma, SAB1400745); anti-β-tubulin, 1:5,000 (Sigma, T5201); anti-ACSS2, 1:2,500 (Cell Signaling Technology, 3658S); anti-acetyl-histone H3, 1:1,000 (Millipore, 06-599); anti-acetyl-histone H4, 1:1,000 (Millipore, 06-598); anti-histone H3, 1:5,000 (Active Motif, 39763); and anti-histone H4, 1:5,000 (Active Motif, 61521). Secondary antibodies were IRDye680LT- and IRDye800CW-conjugated (LI-COR Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!