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Igd fitc

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IgD-FITC is a fluorescently labeled antibody that binds to the immunoglobulin D (IgD) protein. It is used for the identification and analysis of IgD-expressing cells in flow cytometry applications.

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Lab products found in correlation

Igm apc, by Thermo Fisher Scientific (3 mentions) Igm pe, by Southern Biotech (2 mentions) Igd apc cy7, by BioLegend (2 mentions) Cd73 pacificblue, by BioLegend (2 mentions) Cd4 percp, by BD (2 mentions) Cd8a pe, by BioLegend (2 mentions) Cd45 apc cy7, by BD (2 mentions) Igd pe, by Thermo Fisher Scientific (2 mentions) Cd3 fitc, by Thermo Fisher Scientific (2 mentions) Accuri flow cytometer, by BD (2 mentions) Cd45r b220 apc, by Thermo Fisher Scientific (2 mentions) Cd19 pe, by Thermo Fisher Scientific (2 mentions) Cd45 amcyan, by BD (1 mentions) Cd19 pe cy5, by BD (1 mentions) Cd39 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd73 efluor450, by Thermo Fisher Scientific (1 mentions) Cd21 fitc, by Thermo Fisher Scientific (1 mentions) Cd23 apc, by Thermo Fisher Scientific (1 mentions) Cd45 fitc, by BD (1 mentions) Cd19 pe cy7, by BioLegend (1 mentions)

Close spelling variants of this product

IgD (11–26c) FITC (2 citations) Goat F(ab')2 Anti-Human IgD- FITC (2 citations)

8 protocols using igd fitc

1

Multicolor Flow Cytometry Panel

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Antihuman: p-BTK (pY223) PE, CD19 PE-Cy5, IgM PE, CD45 AmCyan and CD45 FITC (Becton Dickinson, USA), CD39 PE-Cy7, CD73 FITC, CD73 eFluor450, CD21 FITC and CD23 APC (eBioscience, USA). Anti-mouse: B220 FITC, CD4 PerCP, CD45 APC-Cy7 (BD-Pharmingen, USA), IgM PE (Southern Biotech, USA) and CD3e eFlour450, CD39 PE-Cy7, IgD FITC, IgM APC (eBioscience, USA), IgD APC-Cy7, CD73 PacificBlue, CD19 PE-Cy7 and CD8a PE (BioLegend, USA). All human mAbs were titrated using normal PBMC to establish optimal dilution.
Jeske S.S., Brand M., Ziebart A., Laban S., Doescher J., Greve J., Jackson E.K., Hoffmann T.K., Brunner C, & Schuler P.J. (2020). Adenosine-producing regulatory B cells in head and neck cancer. Cancer Immunology, Immunotherapy, 69(7), 1205-1216.
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2

Dissecting Murine Lymphoid Cell Subsets

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Lymphoid cells from bone marrow, thymus and spleen were isolated from 8-week old mice. To reveal T cell progenitor subsets in the thymus, single cell suspensions were stained with conjugated antibodies, specific for CD3-FITC (Biolegend), CD4-APC, CD8a-PerCp-Cy5.5, CD25-PE, CD44-APCCy7 (Biolegend), TCRβ-Pacific Blue (Biolegend). To analyse lymphoid subsets in the spleen, single cell suspensions were stained with CD3-FITC, CD4-APC, CD8a-PerCp-Cy5.5, CD19-APCH7, CD45R(B220)-PacificBlue, IgD-PE (eBioscience), IgM-PECy7 (eBioscience). To define the B cell progenitor subsets, suspensions from the bone marrow were stained with IgD-FITC, CD25-PE, IgM-PECy7 (eBioscience), CD45R (B220)-PacificBlue, CD117 (cKit)-APC (eBioscience), CD19-APCH7. Dead cells were excluded from the analysis by propidium iodide staining. Antibodies were purchased from BD Pharmingen unless mentioned otherwise. Samples were measured on a FACS Fortessa® and analysed using FlowJo® software (Version: 10.0.8r1).
Pilzecker B., Buoninfante O.A., Pritchard C., Blomberg O.S., Huijbers I.J., van den Berk P.C, & Jacobs H. (2016). PrimPol prevents APOBEC/AID family mediated DNA mutagenesis. Nucleic Acids Research, 44(10), 4734-4744.
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3

Quantifying B Cell Populations

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To quantify B cell populations in spleen and bone marrow single cell suspensions (106 cells/reaction) were prepared and surface stained in FACS buffer (phosphate-buffered saline (PBS) containing 1% Fetal Bovine Serum (FBS) for 30 minutes at 4°C, and washed once in FACS buffer. B cells were stained using CD45R (B220)-APC or IgD-FITC (eBioscience Inc. (San Diego, CA) and gates established using isotype and fluorochrome-matched controls. Samples were acquired on an Accuri flow cytometer (BD Immunocytometry Systems).
Li Y., Terauchi M., Vikulina T., Roser-Page S, & Weitzmann M.N. (2014). B Cell Production of Both OPG and RANKL is Significantly Increased in Aged Mice. The Open bone journal, 6, 8-17.
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4

Multiparameter Immune Cell Profiling

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Directly conjugated antibodies were acquired from the following: (1) BD Biosciences: CD3-H7APC, CXCR5-Alexa488 (RF8B2), CCR7-Alexa700, IgG-APC, IFN-γ-Alexa700 and IL-21-Alexa647 (3A3-N2.1) (2) Beckman Coulter: CD45RO-ECD and CD27-PC5 (3) Biolegend: CCR7-BV421, CCR6-PE (TG7/CCR6), CCR6-Alexa647 (TG7/CCR6), CD20-BV570, CD150-PE, IL-2-BV605, IL-17a-Cy5.5PerCP and CD154-Cy5PE (4) Invitrogen: CD4-Cy5.5PE, CD27-QD655, CD27-QD605 and CD19-PacBlue (5) Southern Biotech: IgD-FITC and IgD-PE (6) eBioscience: cmaf-eFluor660 (sym0F1), CXCR5-PerCP-efluor710 (MU5UBEE). Biotinylated anti-PD-1 was from R&D and streptavidin-Cy7PE (or QD655) was from Molecular Probes. The following antibodies were conjugated in our lab: CD19-QD705 and CD57-QD565. Quantum dots and Aqua amine viability dye were obtained from Invitrogen.
Boswell K.L., Paris R., Boritz E., Ambrozak D., Yamamoto T., Darko S., Wloka K., Wheatley A., Narpala S., McDermott A., Roederer M., Haubrich R., Connors M., Ake J., Douek D.C., Kim J., Petrovas C, & Koup R.A. (2014). Loss of Circulating CD4 T Cells with B Cell Helper Function during Chronic HIV Infection. PLoS Pathogens, 10(1), e1003853.
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5

Comprehensive Immune Cell Profiling

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All antibodies are from BD Biosciences, except where noted otherwise. CD3-biotin, CD11b-FITC -PE -PE-Cy7 -biotin, CD16/32-PE, CD19-PE, CD24-PE-Cy7, CD34-FITC, CD43-PE, CD48-FITC, CD95-PE, CD150-PE-Cy7, c-Kit-APC -FITC (eBioscience), Sca-1-PE – PE-Cy7 (eBioscience), B220-biotin, FLT3-PE (eBiosciences), Gr-1-FITC -biotin, IgM-APC, IgD-FITC (eBioscience), IL-7R-APC -biotin, TER119-biotin, GL7-FITC and TCR-APC. Cells incubated with biotinylated antibodies were treated with streptavidin conjugated with APC-Cy7 (BD Biosciences). Multipotent progenitors (MPPs) as FLT3+ LSK cells, common lymphoid progenitors (CLPs) as Lin−, IL-7R+ Sca-1lo c-Kitlo; granulocyte macrophage progenitors (GMPs) as Lin−, CD16/32hi, CD34+, Sca1−, c-Kit+; common myeloid progenitors (CMPs) as Lin−, CD16/32lo, CD34+, Sca1−, c-Kit+; and megakaryocyte erythroid progenitors (MEPs) as Lin−, CD34−, CD16/32−, Sca-1−,c-Kit+. Pre-B, pro-B and prepro-B cells were determined by CD24 and CD43 staining of B220+, IgM− bone marrow cells.
Duyvestyn J.M., Taylor S.J., Dagger S.A., Orandle M., Morse HC I.I.I., Thien C.B, & Langdon W.Y. (2014). Dasatinib Targets B-Lineage Cells but Does Not Provide an Effective Therapy for Myeloproliferative Disease in c-Cbl RING Finger Mutant Mice. PLoS ONE, 9(4), e94717.
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6

Comprehensive Immune Profiling of Whole Blood Samples

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To determine the NK cells and lymphocyte subsets in patients, heparin‐anticoagulated whole blood samples were collected and stained with (1) CD3‐PerCP (BD Biosciences, San Jose, CA,), CD4‐BV510 (BD Biosciences, San Jose, CA), CD8‐APC (BD Biosciences, San Jose, CA), CD45RO‐BV421 (BioLegend, San Diego, CA), and CCR7‐PE (BD Biosciences, San Jose, CA); (2) CD4‐APC‐Cy7 (eBioscience, San Diego, CA), CXCR5‐APC (eBioscience, San Diego, CA), TIM‐3‐PerCP (eBioscience, San Diego, CA), TIGIT‐FITC (eBioscience, San Diego, CA), CD226‐PE‐Cy7 (eBioscience, San Diego, CA), PD‐1‐BV510 (BD Biosciences, San Jose, CA), and ICOS‐BV421 (BD Biosciences, San Jose, CA); (3) CD19‐PE (eBioscience, San Diego, CA), CD27‐PE‐Cy7 (eBioscience, San Diego, CA), CD38‐APC (eBioscience, San Diego, CA), CD24‐APC‐Cy7 (eBioscience, San Diego, CA), IgM‐BV421 (eBioscience, San Diego, CA,), and IgD‐FITC (eBioscience, San Diego, CA); and (4) CD3‐FITC (BD Biosciences, San Jose, CA) and CD16 + CD56‐PE (BD Biosciences, San Jose, CA). Cell counting was performed with absolute counting tubes with a certain number of beads (BD Biosciences, San Jose, CA). The samples were measured using FACS Canto II (BD Biosciences, San Jose, CA). Gating analysis was performed with FlowJo V10 (BD Biosciences, San Jose, CA).
Yan L., Cai B., Li Y., Wang M., An Y., Deng R., Li D., Wang L., Xu H., Gao X, & Wang L. (2020). Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID‐19. Journal of Cellular and Molecular Medicine, 24(24), 14270-14279.
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7

Multiparameter Flow Cytometry for Immune Cell Profiling

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The following anti-mouse monoclonal Antibodies (mAbs) were used for flow cytometry: CD25 APC, IgM APC, CD3e Pacific Blue, CD11b Pacific Blue, CD3 FITC, CD8a FITC, IgD FITC, MHC-II FITC, CD39 PE-Cyanin7 (all eBioscience, Waltham, MA, USA); CD4 APC/Cy7, CD21/CD35 APC/Cy7, IgD APC/Cy7, CD73 Pacific Blue, CD8a PE, B220/CD45R PE-Cyanin7, CD19 PE-Cyanin7, F4/80 PE-Cyanin7, Ly6G PerCP/Cy5.5 (all BioLegend, San Diego, CA, USA); CD11c APC, CD45 APC/Cy7, B220/CD45R FITC, CD23 PE, CD44 PE, CD4 PerCP (all BD Biosciences, San Jose, CA, USA); IgM PE (all SouthernBiotech, Birmingham, AL, USA); Ly6C PE, Ter119 PE (all Miltenyi Biotech, Bergisch Gladbach, Germany).
Brand M., Laban S., Theodoraki M.N., Doescher J., Hoffmann T.K., Schuler P.J, & Brunner C. (2020). Characterization and Differentiation of the Tumor Microenvironment (TME) of Orthotopic and Subcutaneously Grown Head and Neck Squamous Cell Carcinoma (HNSCC) in Immunocompetent Mice. International Journal of Molecular Sciences, 22(1), 247.
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8

Quantifying B Cell Populations

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To quantify B cell populations in spleen and bone marrow single cell suspensions (106 cells/reaction) were prepared and surface stained in FACS buffer (phosphate-buffered saline (PBS) containing 1% Fetal Bovine Serum (FBS) for 30 minutes at 4°C, and washed once in FACS buffer. B cells were stained using CD45R (B220)-APC or IgD-FITC (eBioscience Inc. (San Diego, CA) and gates established using isotype and fluorochrome-matched controls. Samples were acquired on an Accuri flow cytometer (BD Immunocytometry Systems).
Li Y., Terauchi M., Vikulina T., Roser-Page S, & Weitzmann M.N. (2014). B Cell Production of Both OPG and RANKL is Significantly Increased in Aged Mice. The Open bone journal, 6, 8-17.
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