KrasLSL-G12D (JAX #008179 (ref. [16 (link)])) (Jackson Laboratory, Bar Harbor, ME, USA), p53flox (JAX #008462 (ref. [40 (link)])), FVB KrasLSL-G12Dp53flox (gift from F. Slack), and Gata6flox (JAX #008196 (ref. [18 (link)])) mice were backcrossed to FVB or C57Bl/6 background for ten generations to obtain FVB and C57Bl/6 KP, KPG, K, and KG lines. Tumors were initiated by intratracheal infection of mice with viral vectors expressing Cre. Additional details are included in Supplementary Table 5 . For syngeneic grafts, 6-week-old C57Bl/6 (JAX #00064) mice were injected subcutaneously with SPC-Cre tumor cells. For delayed expression of shRNAs, 7-week-old Athymic NCr-nu/nu (Charles River NCI #553) mice were injected subcutaneously with SPC-Cre cells containing doxycycline inducible shRNAs. Tumor growth was measured by bioluminescent imaging using an IVIS Spectrum or calipers. All animal studies were approved by the Yale University Institutional Animal Care and Use Committee.
Athymic ncr nu nu
Manufactured by Charles River Laboratories
The Athymic NCr-nu/nu is a laboratory animal model that lacks a functional thymus gland, resulting in T cell deficiency. This model is commonly used in immunological and oncological research.
Automatically generated - may contain errors
Lab products found in correlation
Ncg nod prkdcem26cd52il2rgem26cd22 njucrl, by Charles River Laboratories (2 mentions)
Balb canncr female, by Charles River Laboratories (2 mentions)
Kraslsl g12d, by Jackson ImmunoResearch (1 mentions)
P53flox, by Jackson ImmunoResearch (1 mentions)
B6.129s2 cd40lgtm1imx j, by Jackson ImmunoResearch (1 mentions)
Skh 1 elite mice crl skh1 hrhr, by Charles River Laboratories (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Balb cj, by Jackson ImmunoResearch (1 mentions)
7 protocols using athymic ncr nu nu
1
Genetically Engineered Mouse Models for Lung Cancer
2
Xenograft Tumor Models in Mice
3
Athymic Nude Mice for Cancer Research
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Female nude mice (Athymic Ncr-nu/nu) were purchased from Charles River Laboratories Inc (Frederick, MD). All mice were housed in the animal facility at Frederick National Laboratory for Cancer Research (Frederick, MD) and were used at the age of 10 weeks. Animal care was provided in accordance with procedures outlined in the Guide for Care and Use of Laboratory Animals (National Research Council, 1996, National Academy Press, Washington D.C.).
4
Establishment of PSMA-Expressing Prostate Cancer Xenografts
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PC3(−) (wildtype human prostate cancer cell line, PSMA negative) and PC3(+) (transfected with human PSMA) were provided by Dr. Hisataka Kobayashi [55 (link),57 (link)]. Cell lines were grown at 37 °C in 5% CO2 in RPMI-1640 supplemented with 10% FBS, 2 mM L-glutamine and Pen/Strep/Amphotericin B. PC3(+) and PC3(−) cell suspensions [2 × 106 cells; PBS:Matrigel (70:30)] from in vitro cell culture were subcutaneously implanted (right shoulder) into athymic mice (Athymic NCr- nu/nu, Charles River Laboratory, 4 weeks old) for use as positive and negative controls, respectively, for in vitro or in vivo studies. When tumors reached the appropriate size (>100 mg) the xenograft mice were used for in vivo biodistributions and imaging studies or the tumors were excised and further processed to obtain membrane preparations for in vitro assays as described previously [58 (link)].
5
Xenograft Tumor Models in Mice
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Human cell lines were injected in Nude female Athymic NCr-nu/nu from Charles Rivers (HEp3 model) or NCG (NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl) strain from Charles River Labs (MDA-MB-231) between 8 and 12 weeks old. Murine cell lines were injected in BALB/cAnNCR female from Charles Rivers between 8 and 12 weeks old. Each time mice were anesthetized and lubricant Lubrifesh Major (reference NDC 0904–6488-38) was applied to their eyes to prevent drying.
6
Murine Models for Immunological Studies
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6–8 weeks old female C57BL/6J (strain code: 000664), BALB/cJ (strain code: 000651), Rag1 Knock-out (B6.129S7-Rag1tm1Mom/J), CD4- Knockout (B6.129S2-Cd4 tm1Mak/J) and CD40 ligand knockout (B6.129S2-Cd40lg tm1Imx/J) were purchased from Jackson Laboratories Inc. Immunodeficient athymic Nude mice (Athymic NCr-nu/nu), SCID mice (NCI SCID/Ncr, BALB/c background) and immuno-competent SKH-1 elite mice (Crl:SKH1-Hrhr) were obtained from Charles River, Inc. IFNAR knockout mice were a kind gift from Dr. G. Cheng (University of California, Los Angeles, CA) All animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and with the prior approval of the Animal Care and Use Committee of Johns Hopkins University (MO12M223).
7
In Vivo Xenograft Tumor Modeling
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Studies were conducted in compliance with US guidelines for the care and use of laboratory animals and were approved by the Institutional Animal Care and Use Committee of Yale University. 5 × 105 HeLa/iCas9-c1 cells carrying control sgRNA or KDM5A/B/C sgRNAs were subcutaneously injected into the flanks of the 6-week-old female nude mice (Athymic NCR-nu/nu, Charles River Laboratories). After the tumors reached the size of ∼0.5 cm3, the animals were fed with normal chow or chow containing 625 mg/kg DOX for 5 days before euthanizing and harvesting tumor tissues for western blot analyses.
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