Latent inhibition was assessed using a fear conditioning paradigm (Smith et al., 2007 (link)). Animals were placed in test chambers (Coulbourn Instruments, Allentown, PA) with a speaker and grid floor connected to a shock source, and a video camera mounted in the ceiling connected to Coulbourn Freeze Frame software. Each test chamber was positioned inside a sound-attenuating enclosure. Pair-B was the PE (pre-exposed) group. On day 1, these animals were placed in the test chambers for acclimation followed by 30 tones (82 dB, 2 kHz, 30 s duration) separated by 30 s inter-stimulus intervals which was then followed by 3 tone-footshock pairings with the shock (0.5 mA) occurring during the last 1 s of the tone interval. On Day-2, rats were returned to the test chambers to assess contextual conditioning for 6 min during which immobility intervals of >4 s were scored as freezing. On Day-3, rats were returned to the test chambers with a different floor texture and tested for 6 min; the first 3 min with no-tone and the last 3 min with tone and again scored for bouts of immobility.
Test chamber
Manufactured by Harvard Apparatus
Sourced in United States
Test chambers are enclosed spaces designed to simulate and control various environmental conditions for research and testing purposes. They are used to evaluate the performance, durability, and behavior of materials, products, or living organisms under specified environmental parameters such as temperature, humidity, pressure, or atmospheric composition.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using test chamber
1
Latent Inhibition and Fear Conditioning
2
Fear Conditioning Protocol for Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To facilitate habituation to the experimenter/non-associative aspects of fear conditioning, mice were transported to the testing room and handled for 30–60 s daily for 3 days before testing, as previously reported22 (link),34 (link). On the training and test days, mice were transported to the testing room and placed into test chambers (9.8 in boxes; designed for mice) equipped with an electrified grid floor (Coulbourn Instruments, Holliston, MA, USA). During training, mice were given 2 min to explore the apparatus before receiving a single foot shock (0.5 mA, 2 s), followed by an additional minute of exploration. Memory was assessed 24 h later by replacing the mouse into the training apparatus without shock and measuring freezing behavior for 3 min. Freezing was recorded with a camera placed directly in the chamber ceiling and scored by automated software (FreezeFrame, Coulbourn Instruments).
3
Social Interaction Dynamics in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For social interaction experiments, male mice were introduced in a test chamber (Coulbourn Instruments) where they underwent three separate bouts of interaction with a novel ovariectomized C57BL/6NJ female mice (8–12 weeks old). Each bout lasted approximately 2 min. For optogenetic experiments male subjects received a total of six interaction bouts, three in the presence and three in the absence of laser stimulation.
4
Social Interaction Dynamics in Mice
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!