Dna cloning kits

All chemical reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO, United States). Oligonucleotides and gene synthesis reagents were provided by Macrogen (Seoul, South Korea). Restriction endonucleases, polymerases, and DNA cloning kits were purchased from New England Biolabs (Ipswich, MA, United States). DNA preparation and manipulation techniques were carried out according to standard protocols for molecular biology. The kits for PCR product purification, gel extraction, and plasmid preparation were purchased from Promega (Madison, WI, United States). Profinia^TM purification kits and all materials for SDS-PAGE were purchased from Bio-Rad (Hercules, CA, United States).

All chemical reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA). Restriction endonucleases, DNA cloning kits, pUC19 plasmid, and E. coli DH5α cells were purchased from New England Biolabs (Ipswich, MA, USA). The Diversify PCR Random Mutagenesis kit and QuikChange II XL Site-Directed Mutagenesis kit were purchased from Clontech (Mountain View, CA, USA) and Agilent Technologies (San Diego, CA, USA), respectively. Plasmid DNA isolation and DNA extraction were conducted using plasmid preparation kits (Promega, Madison, WI, USA). Oligonucleotides (Supplementary Table 5) were synthesized and sequenced by Macrogen (Daejeon, Korea).

All the reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA). The restriction endonucleases, polymerases, and DNA cloning kits were obtained from New England BioLabs (Ipswich, MA, USA), and the DNA purification kits were from Promega (Madison, WI, USA). Oligonucleotides and synthesized genes were provided by Macrogen (Seoul, Korea) (Table S1). The DNA preparation and manipulation techniques were performed according to standard molecular biology protocols.