Anti cd86 pe

For further investigating the maturation level of BMDC after treated with APG@OVA NPs, the expression levels of surface molecules (CD 40, CD 80, CD83, CD86, and MHC-II) were evaluated by FACS. After being treated with APG@OVA NPs, BMDCs were washed with PBS and stained with anti-CD40-FITC (BD Pharmingen), anti-CD80-FITC, anti-CD83-PE, anti-CD86-PE and anti-MHC-II-FITC antibodies (MultiSciences) for 20 min at room temperature. The cellular fluorescence of these BMDCs was measured by FACS analysis (BD FACS) after being washed with PBS.

Penicillin, fetal bovine serum (FBS), DMEM/ F12 medium, and PBS were purchased from GE™ Hyclone. APS was purchased from Beijing Solarbio Science & Technology Co., Ltd. ATP was purchased from Shanghai Zeye Biological Technology Co., Ltd. Rat ATP ELISA Kit and rat ADP ELISA Kit were purchased from Beijing winter song Boye Biotechnology Co. Ltd. EBD was purchased from Real-Times (Beijing) Biotechnology Co., Ltd. EDTA antigen repair solution was purchased from Servicebio. Antibodies: anti-GAPDH (KC-5G5, KangChen Bio-tech), anti-P2X7R (BA2808, Boster Biological Technology Co., Ltd.), anti-CD163 (ab182422, Abcam), anti-CD206 (sc-58,986, Santa Cruz), anti-CD86 (NBP2-25,208, Novus), anti-HLA-DR (MA5-32,232, Invitrogen), anti-CD163-PE (85–12-1639-41, eBioscience), anti-CD206-APC (85–17-2069-41, eBioscience), anti-CD86-PE (70-AR08604-100, MultiSciences), and anti-HLA-DR-APC (70-AH0HD05-20, MultiSciences).