Samples were lysed in lysis buffer (50 mM Tris (pH 7.4), 150 mM KCl, 1 mM EDTA, 1% NP-40, 5 mM NAM, 1 mM sodium butyrate, protease and phosphatase inhibitors). Proteins were separated by SDS–PAGE and transferred onto nitrocellulose or polyvinylidene difluoride membranes. Blocking (30 min) and antibody incubations (overnight) were performed in 5% BSA in TBST. YAP1 (Santa Cruz sc101199, 1:1000), phospho-YAP1Y357 (Abcam ab62751, 1:1000), ACTIN (Santa Cruz sc47778, 1:1000), CXCL10 (RD system AF-466-NA, 1:1000), CCL2 (Novus Biologicals NBP2-22115, 1:1000), COL1A2 (Santa Cruz, 1:1000).
Phospho yap1y357
Manufactured by Abcam
Phospho-YAP1Y357 is a laboratory product that detects the phosphorylation of the YAP1 protein at the tyrosine 357 residue. This product can be used in various research applications to study the regulation and activity of the YAP1 protein, which is involved in cellular processes such as proliferation and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Sc 101199, by Abcam (1 mentions)
Col1a2, by Santa Cruz Biotechnology (1 mentions)
Cxcl10, by R&D Systems (1 mentions)
Bradford dye reagent, by Bio-Rad (1 mentions)
Bis tris gel, by Thermo Fisher Scientific (1 mentions)
Phospho egfr y1068, by Abcam (1 mentions)
Ripa buffer, by Cell Signaling Technology (1 mentions)
Protease and phosphatase inhibitor, by Roche (1 mentions)
2 protocols using phospho yap1y357
1
Protein Detection in Cell Lysates
2
Comprehensive Western Blot Analysis
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Cells were washed with ice-cold PBS and lysed with RIPA buffer (Cell Signaling Technology) supplemented with protease and phosphatase inhibitors (Roche). Protein levels were quantified with Bradford dye reagent (Bio-Rad), and equal amounts were loaded for SDS/PAGE using precast Bis-Tris gels (Invitrogen), followed by transfer to polyvinylidene difluoride membranes. Membranes were blotted with the following antibodies according to the supplier’s recommendations and were all obtained from Cell Signaling Technology unless otherwise noted: phospho-EGFR Y1068 (no. 3777), EGFR E746-A750del specific (no. 2085), phospo-MET Y1234/1235 (no. 3777), MET (no. 8198), phospho-SFK (no. 6943), YES (no. 3201), phospho-YAP1 Y357 (no. ab62751; Abcam), YAP1 (no. 14704), phospho-AKT S473 (no. 4060), AKT (no. 4691), phospho-ERK T202/Y204 (no. 4370), ERK (no. 4695), phospho-ERBB3 Y1197 (no. 4561), ERBB3 (no. 12708), and GAPDH (no. 2118). Human phosphokinase (no. ARY003B; R&D Systems) and human phospho-RTK (no. ARY001B; R&D Systems) array kits were used according to the manufacturer’s protocols.
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