Cyp19a1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Cyp19a1 is a gene that encodes the enzyme aromatase, which is responsible for the conversion of androgens to estrogens. This enzyme plays a crucial role in the regulation of estrogen levels in the body. Cyp19a1 is commonly studied in research applications involving the investigation of hormonal pathways and reproductive biology.

Automatically generated - may contain errors

Lab products found in correlation

Taqman fast advanced master mix, by Thermo Fisher Scientific (1 mentions) Prism 7900ht sequence detection system, by Thermo Fisher Scientific (1 mentions) Cyp11a1, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Taqman universal master mix, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using cyp19a1

Quantifying mRNA Expression in Rat Tissues

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mRNA levels were analyzed using real-time PCR with the TaqMan Fast Advanced Master Mix (Applied Biosystems) and the Prism 7900HT Sequence Detection System (Applied Biosystems) and normalized to that of the endogenous control Gapdh. All oligonucleotide primers and fluorogenic probe sets were manufactured by Applied Biosystems (Fshr, Rn01648507_m1; Bmp2, Rn00567818_m1; Bmp6, Rn00432095_m1; Cyp19a1, Rn00567222_m1; Hsd17b, Rn00563388_m1; Star, Rn00580695_m1; Cyp11a1, Rn00568733_m1; Hsd3b, Rn01789220_m1, and Gapdh, Rn01775763_g1).

Yoshita S., Osuka S., Shimizu T., Fujitsuka N., Matsumoto C., B.a.y.a.s.u.l.a., Miyake N., Muraoka A., Nakanishi N., Nakamura T., Goto M, & Kajiyama H. (2023). Unkeito promotes follicle development by restoring reduced follicle-stimulating hormone responsiveness in rats with polycystic ovary syndrome. Frontiers in Endocrinology, 14, 1228088.

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Quantitative Gene Expression Analysis in Neurons

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Total RNA from primary neurons was isolated as previously described (75 (link)). Mouse hippocampi were homogenized in the RLT buffer from RNeasy Mini Kit (Qiagen, 74106) with a tissue grinder gun and further processed according to the manufacture protocol for RNA extraction. Retro transcription was done using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368814). mRNA copy numbers relative to Gapdh mRNA levels were measured by real-time PCR using Taqman Universal MasterMix (Applied Biosystems, USA) and the following primers: Esr1, Esr2, Arc, Bdnf, Cyp19a1, Sdr5a1, Hsd17b10, Hsd17b1, Rara, Cyp26b1, Srepb1, Rxrg, and Gapdh (Applied Biosystems, USA).

Latorre-Leal M., Rodriguez-Rodriguez P., Franchini L., Nikolidakis O., Daniilidou M., Delac L., Varshney M.K., Arroyo-García L.E., Eroli F., Winblad B., Blennow K., Zetterberg H., Kivipelto M., Pacciarini M., Wang Y., Griffiths W.J., Björkhem I., Matton A., Nalvarte I., Merino-Serrais P., Cedazo-Minguez A, & Maioli S. (2024). CYP46A1-mediated cholesterol turnover induces sex-specific changes in cognition and counteracts memory loss in ovariectomized mice. Science Advances, 10(4), eadj1354.

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