Histone/DNA complexes (Millipore Sigma) and citrullinated histone H3 (Cayman Chemicals, Ann Arbor, MI) were determined by the ELISA methods according to the manufacturer’s instructions. Arbitrary units equal to the ratio of optical density of a patient plasma sample to the optical density of a pooled human plasma.
Citrullinated histone h3
Manufactured by Cayman Chemical
Sourced in United States
Citrullinated histone H3 is a laboratory reagent that can be used to detect post-translational modifications of histones. Histones are nuclear proteins that play a crucial role in the organization and regulation of chromatin within eukaryotic cells. Citrullination is a specific type of post-translational modification that involves the conversion of arginine residues to citrulline. This product can be utilized in various research applications to study the role of histone citrullination in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions)
Il 12p40, by R&D Systems (1 mentions)
Il 12p70, by R&D Systems (1 mentions)
Alpha 1 antitrypsin, by Abcam (1 mentions)
Vegf a, by R&D Systems (1 mentions)
Mmp 1, by R&D Systems (1 mentions)
Quant it picogreen dsdna assay, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Cell Signaling Technology (1 mentions)
4 protocols using citrullinated histone h3
1
Quantifying Citrullinated Histone H3
2
Multiplex Biomarker Profiling Protocol
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Luminex, model L200 (Millipore, Massachusetts, USA) was used to evaluate serum levels of, IL-8, IL-12p40, IL-12p70, VEGF-A, MMP-1, and-8 (R&D Systems, Minneapolis-USA). The results were presented in median fluorescence intensity (MFI) units.
Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum levels of citrullinated histone H3 (Cayman, Michigan-USA) and alpha-1-antitrypsin (Abcam, Cambridge-USA).
Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum levels of citrullinated histone H3 (Cayman, Michigan-USA) and alpha-1-antitrypsin (Abcam, Cambridge-USA).
3
Quantifying Histone-DNA Complexes and cfDNA
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Plasma levels of histone/DNA complex (Millipore Sigma) and citrullinated histone H3 (Cayman Chemicals, Ann Arbor, MI, USA) were determined by the commercial ELISA kits according to the manufacturers' instructions. Cell-free DNA was determined using the Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) according to the manufacturer's instructions.
4
Quantifying Cell Death and NET Formation
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Normal (uninfected) and infected corneas, and PMN cell lysates were collected from each treatment group. Samples were homogenized in RIPA buffer (Cell Signaling Technology, Danvers, MA, USA) together with a protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Digested samples were centrifuged at 5000× g for 5 min, and an aliquot of each supernatant was assayed in triplicate for cell death detection (Sigma-Aldrich, St. Louis, MO, USA) and citrullinated histone H3 (Cayman Chemical, Ann Arbor, MI, USA) per the manufacturer’s instructions. The absorbance of cell death detection was measured at 405 nm, and the enrichment factor (EF) was calculated based on the provided formula. The reported sensitivity of citrullinated histone H3 is 0.3 ng/mL and data are presented as average ng/mL ± SD.
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