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Anti ntb a nt7

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Anti-NTB-A (NT7) is a mouse monoclonal antibody targeting the NTB-A protein. NTB-A is an immunoglobulin-like receptor that regulates the activation and function of natural killer cells and other lymphocytes.

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Lab products found in correlation

Anti nkp30 p30 15, by BioLegend (2 mentions) Anti ifn γ b27, by BioLegend (1 mentions) Anti nkg2d 1d11, by BioLegend (1 mentions) Anti dnam 1, by R&D Systems (1 mentions) Expre35s35s protein labeling mix, by PerkinElmer (1 mentions) Roswell park memorial institute (rpmi), by Merck Group (1 mentions)

2 protocols using anti ntb a nt7

1

Evaluating iNK Cell Cytotoxicity

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iNK cells were incubated for 30 minutes at 37°C with the following monoclonal antibodies: anti-NKG2D (1D11) at 20 μg/ml, anti-NTB-A (NT7) at 5 μg/ml, anti-NKp30 (P30–15) at 10 μg/ml, anti-LFA-1 (HI111) at 10 μg/ml (all from Biolegend), and anti-DNAM-1 at 10 μg/ml (102511) (R&D Systems) alone or in various combinations. iNK cells were then co-cultured with OVCAR8 cells for 4 hours in B0 media and analyzed by flow cytometry for intracellular IFN-γ production using a fluorescently conjugated anti-IFN-γ (B27) (Biolegend) antibody.
Cichocki F., Bjordahl R., Gaidarova S., Mahmood S., Abujarour R., Wang H., Tuininga K., Felices M., Davis Z.B., Bendzick L., Clarke R., Stokely L., Rogers P., Ge M., Robinson M., Rezner B., Robbins D.L., Lee T.T., Kaufman D.S., Blazar B.R., Valamehr B, & Miller J.S. (2020). iPSC-derived NK cells maintain high cytotoxicity and enhance in vivo tumor control in concert with T cells and anti-PD-1 therapy. Science translational medicine, 12(568), eaaz5618.
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2

Redirected Cytotoxicity Assay for NK Cells

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Cytotoxicity through individual receptors was measured in a redirected lysis assay. P815 target cells were labeled for 3 hours with EXPRE35S35S Protein Labeling Mix (Perkin Elmer, Inc.) in cysteine/methionine-free RPMI (Sigma). Target cells were washed 3 times in complete RPMI and dispensed into 96-well round bottom plates at 5000 cells/well. Lentiviral-transduced NK92 cells were harvested and mixed with target cells at the indicated effector:target ratio in duplicate unless otherwise stated in the presence of IL-2. The indicated antibodies were added to the cellular mixture for the duration of the 4 hour cytotoxicity assay. The antibodies used to induce cytotoxicity were as follows: anti-NKp30 (P30.15) and anti-NTB-A (NT-7) were obtained from Biolegend; anti-CRACC (162) and anti-CD58 (MEM-63) were obtained from Abd Serotec; anti-CD2 (X53) and anti-CD244 (C1.7) were purified in our laboratory. The numbers of independent experiments were: SAP – 2, EAT2 – 3, PLCγ1 - 4, and PLCγ2 - 2.
Wilson T.J., Garner L.I., Metcalfe C., King E., Margraf S, & Brown M.H. (2014). Fine Specificity and Molecular Competition in SLAM Family Receptor Signalling. PLoS ONE, 9(3), e92184.
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