SPECT/CT studies were performed using the MILabs U-SPECT- II (Advanced Molecular Vision). LNCaP95 and PC-3 tumor-bearing mice (20-25 g) were anesthetized with isoflurane inhalation at 2% and placed on a heating pad to maintain body temperature. The mice were then injected with 123I-EPI-002 (37–74 MBq; 1–2 mCi, in 200 μl of 30% PEG solution) via the tail vein. A competition experiment was performed by coinjection of the radiotracer with 50 mg/kg of EPI-002 in a total volume of 200 μl of 30% PEG solution. SPECT scans were acquired over 30 minutes and 2 frames at 15 minutes each using 1.0-mm multi-pinhole collimator, after which CT scans were performed for anatomic reference (parameters; 60 kV, 600 μA). SPECT imaging data were reconstructed using MI Labs reconstruction software (2 subsets, 30 iterations). PMOD software was used to analyze and view the images, and a Gaussian filter was applied after reconstruction.
Reconstruction software
Manufactured by MILabs
The Reconstruction software is a core component of MILabs' laboratory equipment. It is designed to process and reconstruct data obtained from various imaging modalities. The software's primary function is to generate high-quality images from the acquired data, enabling researchers and scientists to analyze and interpret their findings effectively. The Reconstruction software is an essential tool for researchers working in fields that require advanced imaging techniques.
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Lab products found in correlation
4 protocols using reconstruction software
1
In Vivo Imaging of Prostate Cancer Xenografts
2
In Vivo Renal Function Monitoring
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To monitor the renal function in vivo, mice in the tolerability study underwent SPECT imaging using 99mTc-labeled dimercaptosuccinic acid (DMSA) at baseline, 10 weeks p.i., and 16 weeks p.i. Preparation of [99mTc] Tc-DMSA (99mTc-DMSA) was performed as per the manufacturer’s protocol (Curium Netherlands B.V., Petten, the Netherlands). In short, 740–1100 MBq of pertechnetate was added to 1.2 mg of DMSA and incubated for 15 min at 25 °C. Mice received an intravenous tail injection of 20 MBq 99mTc-DMSA in 200 µL saline 2 h prior to SPECT imaging. The SPECT scans were acquired with the U-SPECT-II/CT (MILabs, Utrecht, the Netherlands) using a 1.0 mm diameter pinhole mouse high sensitivity collimator and acquisition time of 15 min. Image reconstruction was performed with MILabs reconstruction software using a 16-subset expectation maximization algorithm with a voxel size of 0.2 mm and 3 iterations. Quantification of renal uptake of 99mTc-DMSA was performed by drawing volumes of interest (VOIs) around the kidneys. The radioactivity was corrected for decay and volume of the VOI, which resulted in a percent injected activity per gram kidney tissue, assuming a tissue density of 1.0 g/cm3.
3
SPECT/CT Imaging in Rodent Studies
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SPECT/CT scans were acquired for either 1
h at 24 or 48 h post injection or for 1.5 h at 96 h post injection
using a U-SPECT/CT-II (MILabs, Utrecht, The Netherlands). Images were
acquired using a 1 mm diameter pinhole ultrahigh sensitivity mouse
collimator or a 1 mm diameter pinhole rat collimator (n = 1). SPECT scans were followed by CT scans (65 kV, 615 μA).
All SPECT scans were reconstructed with 3 iterations and 16 subsets
and a voxel size of 0.4 mm (MILabs reconstruction software). SPECT
images were made with VivoQuant software. The tissues from the mice
that underwent the SPECT/CT scans were also used for the ex vivo biodistribution
as described above after scanning.
h at 24 or 48 h post injection or for 1.5 h at 96 h post injection
using a U-SPECT/CT-II (MILabs, Utrecht, The Netherlands). Images were
acquired using a 1 mm diameter pinhole ultrahigh sensitivity mouse
collimator or a 1 mm diameter pinhole rat collimator (n = 1). SPECT scans were followed by CT scans (65 kV, 615 μA).
All SPECT scans were reconstructed with 3 iterations and 16 subsets
and a voxel size of 0.4 mm (MILabs reconstruction software). SPECT
images were made with VivoQuant software. The tissues from the mice
that underwent the SPECT/CT scans were also used for the ex vivo biodistribution
as described above after scanning.
4
Longitudinal microCT Imaging of Mouse Hindlimbs
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Longitudinal imaging of the hindlimbs of the mice was performed with a high-resolution microCT (U-CTHR, MILabs, Netherlands). The x-ray tube was operated at 50-kVp source voltage and 210-mA source current. Images were acquired at a step angle of 0.375° with two projections per step (75-ms exposure time), over a range of 360° and with 8.5-μm voxel size. Two aluminum filters with 100- and 400-μm thickness were used. The scan region included the entire femur and tibia of both hindlimbs and was determined on an x-ray scout view. To prevent motion artifacts and ensure reproducibility, the anesthetized mice were positioned using an animal bed with the hindlimbs restrained. The scans were reconstructed using the MILabs Reconstruction software, and a projection filter (Hann) was applied in the process to limit blurring. The scanner was calibrated before every scan using the internal calibration system. The animals were scanned with the microCT for a reference scan (day 0, 12-week-old). After 17 days, the animals were scanned twice per week up until maximum 38 days (on days 17, 20, 24, 27, 31, 34, and 38) (fig. S4A). Anesthetized mice were euthanized by cervical dislocation. The hindlimbs were harvested and fixed with 4% paraformaldehyde in PBS over night at 4°C and stored in PBS until further processed.
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