Ab202584

Kidney tissues were collected and fixed in 4% paraformaldehyde for 24 h. After gradient dehydration, the kidney tissues were embedded in paraffin and finally cut into 3-μm sections. The HE staining was performed with a Hematoxylin and Eosin Staining Kit (Beyotime, Shanghai, China). Referring to the evaluation criteria from previous literature, we scored the pathological conditions of the glomerulus and interstitial cells by selecting different visual fields. Morphometric analyses of the glomerulus and renal tubules were determined using ImageJ. For immunostaining, the sections were incubated with anti-Fhl2 antibody (ab202584, Abcam, Cambridge, UK), anti-Lama2 antibody (ab11576, Abcam, Cambridge, UK), anti-DPP4 antibody (GB114937, Servicebio, Wuhan, China), anti-Fn1 antibody (GB114491, Servicebio, Wuhan, China) and pAKT (4060S, Cell Signaling Technology, Boston, MA, USA). After incubation with secondary antibodies for 2 h, the sections were sealed with antifade mounting medium with DAPI (041221210930, Beyotime, Shanghai, China). Images were acquired by a Zeiss AX10 microscope (Carl Zeiss, Weimar, Germany).

Tissue sections were incubated with anti-Fhl2 antibody (1:400, ab202584, Abcam, Cambridge, UK) and anti-Lama2 antibody (1:400, ab11576, Abcam, Cambridge, UK) at 4 °C overnight. After incubation with secondary antibodies for 2 h, the sections were sealed with antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, 041221210930, Beyotime, Shanghai, China). Images were acquired by a Zeiss AX10 microscope (Carl Zeiss, Weimar, Germany).