Ivisbrite d luciferin potassium salt

The distribution of the luciferase transgene was determined by the level of bioluminescence from living animals after substrate injection. At each timepoint, animals were given a 150 mg/kg split dose of IVISbrite™ D-Luciferin Potassium Salt (PerkinElmer) in Phosphate-Buffered Saline pH 7.4 (Thermo Fisher Scientific) by intraperitoneal injection. Image acquisition and analysis were performed using the IVIS® Spectrum and the manufacturer’s software (PerkinElmer).

To verify the location of luciferase-expressing lesions detected with MR imaging, immediately after the last MR imaging session, mice were injected intraperitoneally with IVISbrite D-Luciferin potassium salt in PBS (100 µL of 300 mg/mL, Perkin Elmer, Boston, MA, USA). Ten minutes after injection, mice were euthanized and collected tissues were subjected to bioluminescence imaging (BLI) using an IVIS Spectrum imaging system (Perkin Elmer, Hopkinton, MA, USA). BLI was performed with the following settings: exposure time, 30 s; f number, 8; binning factor, 2. All images were processed using the Living Image Software (version 4.5.2, Perkin Elmer, Hopkinton, MA, USA).