420 microscope

Manufactured by Philips

The -420 microscope is a laboratory instrument designed for magnified observation of small samples. It utilizes a system of lenses to provide high-resolution images of specimens. The core function of the -420 microscope is to enable detailed visual examination and analysis of various materials and samples.

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Lab products found in correlation

Empyrean x ray powder diffractometer, by Malvern Panalytical (1 mentions) Zetasizer zs, by Malvern Panalytical (1 mentions) Vertex v70 ft ir spectrometer, by Bruker (1 mentions)

Close spelling variants of this product

420 electron microscope (5 citations) 420 Transmission Electron Microscope (3 citations)

2 protocols using 420 microscope

Comprehensive Nanomaterial Characterization

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Scanning electron microscopy (SEM) images were obtained on a field-emission scanning electron microscope (NanoSEM 630, NOVA). Transmission electron microscopy (TEM) images were conducted with a Philips-420 microscope at an acceleration voltage of 120 kV. The high angle annular dark field (HAADF) images was obtained by a scanning transmission electron microscopy TITAN (FEI Titan3 G2). The zeta potential was measured by the Malvern Zetasizer ZS. Fourier transform infrared spectra were obtained by a Bruker Vertex V70 FT-IR spectrometer scanned from 400 to 4000 cm⁻¹. The powder X-ray diffraction (PXRD) patterns were performed by a PANalytical Empyrean X-ray powder diffractometer with Cu target (45 kV, 40 mA) from 20 to 70 degrees. The magnetization measurement was collected by a superconducting quantum interface device (SQUID) magnetometer at 300 K.

Yu X., Cheng G, & Zheng S.Y. (2016). Synthesis of Self-Assembled Multifunctional Nanocomposite Catalysts with Highly Stabilized Reactivity and Magnetic Recyclability. Scientific Reports, 6, 25459.

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Negative Staining and Electron Microscopy of Protein Assemblies

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Negative stain samples were prepared by adding 10 μL of assembled protein to a carbon-coated grid. The sample was removed and the grid stained with 5 drops of 2% uranyl acetate. Samples for embedding and sectioning were prepared as described previously.^{20 (link)} Assembled tubes were fixed in 1% glutaraldehyde and centrifuged, and pellets were further fixed in 3% glutaraldehyde and 0.2% tannic acid. They were then stained with 1% osmium tetroxide for 30–60 min, followed by block staining with 2% uranyl acetate. The samples were then dehydrated and embedded in Araldite resin. Silver and gray sections (50–70 nm) were collected for observation by EM. Electron microscopy was performed using a Philips 420 microscope, and images were collected at 49000× magnification unless otherwise stated.

Housman M., Milam S.L., Moore D.A., Osawa M, & Erickson H.P. (2016). FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings. Biochemistry, 55(29), 4085-4091.

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