Total proteins were isolated from cultured cells by using a lysis buffer. Then equal amounts of protein lysates were separated by SDS-page gel electrophoresis. After transferring onto a PVDF membrane, protein was incubated with primary antibodies against Fgf21, Sirt1, Troponin I, t-CamKII, Calpian 1, NOX2, NOX4, GAPDH (Abcam), Nrf2, MHC, SOD2, UCP3, p-RyR2, β-Klotho (Abclonal), ox-CamKII (GeneTex), RyR2, FgfR1 (Proteintech), L-type calcium channel α1C-subunit (LCC) (Affinity), α-SMA, Collagen-1A1, CTGF, and Tubulin (Servicebio). Then they were incubated with a secondary antibody conjugated to HRP (1:5,000) for 1 h. Signals of proteins were identified by chemiluminescence and quantified by densitometry.
T camkii
Manufactured by Abcam
Sourced in United States
T-CamKII is a protein that acts as a calcium sensor. It plays a crucial role in the regulation of various cellular processes, including neuronal signaling, gene expression, and synaptic plasticity.
Automatically generated - may contain errors
Lab products found in correlation
Sirt1, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Fgf21, by Abcam (1 mentions)
Ox camkii, by GeneTex (1 mentions)
α sma, by Wuhan Servicebio Technology (1 mentions)
Bcl 2, by Abcam (1 mentions)
Streptozotocin (stz), by MedChemExpress (1 mentions)
Tween 20, by Avantor (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Avantor (1 mentions)
Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions)
Phenylmethylsulfonyl fluoride, by Avantor (1 mentions)
Radioimmunoprecipitation assay buffer, by Avantor (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Cell Signaling Technology (1 mentions)
Sodium dodecyl sulfate sds, by Avantor (1 mentions)
Trpc6, by Abcam (1 mentions)
2 protocols using t camkii
1
Protein Expression Profiling in Cultured Cells
2
Diabetic Nephropathy Pathogenic Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dimethyl sulfoxide (Sigma-Aldrich, United States), high-sugar Dulbecco’s modified Eagle’s medium (HyClone, United States), low-sugar Dulbecco’s modified Eagle’s medium (HyClone), Cell Counting Kit-8 (CCK-8; Beyotime, China), fetal bovine serum (Amresco, United States), radioimmunoprecipitation assay buffer (Amresco), protein concentration detection kit (Amresco), phenylmethyl sulfonyl fluoride (Amresco), cocktail protease inhibitor (Amresco), sodium dodecyl sulfate (SDS; Amresco), STZ (MedChemExpress, United States), KN-93 (MedChemExpress), Tween-20 (Amresco), SAR (Sigma-Aldrich), primary antibodies against TRPC6 (Abcam, United States), CC3 (Abcam), T-CAMKII (Abcam), P-CAMKII (Signalway Antibody, United States), Bcl-2 (Abcam), glyceraldehyde 3-phosphate dehydrogenase (Cell Signaling Technology, United States), Bax (Cell Signaling Technology), PCNA (Abcam), CyclinD1 (Cell Signaling Technology), and sheep anti-mouse secondary antibody (Abcam) were used in this study.
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