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Mouse rat leptin elisa

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The Mouse/Rat Leptin ELISA is a quantitative sandwich enzyme-linked immunosorbent assay used for the measurement of leptin concentrations in mouse and rat samples. It is a reliable and accurate method for the determination of leptin levels.

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Lab products found in correlation

Labassay triglyceride, by Fujifilm (1 mentions) Labassay nefa, by Fujifilm (1 mentions) Heparin choay, by Sanofi (1 mentions) Free fatty acid fluorometric assay kit, by Cayman Chemical (1 mentions) Glycerol colorimetric assay kit, by Cayman Chemical (1 mentions) Accutrend, by Roche (1 mentions) Nefa c kit, by Fujifilm (1 mentions) Mouse rat insulin kit, by Mesoscale (1 mentions)

Close spelling variants of this product

Leptin (18 citations) Rat Leptin ELISA (2 citations) Mouse leptin ELISA (2 citations) Rat leptin (2 citations) Mouse/rat leptin ELISA kit (2 citations) Mouse and Rat Leptin Elisa kit (2 citations)

6 protocols using mouse rat leptin elisa

1

Plasma lipid and leptin analysis

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Plasma triglyceride and non-esterified fatty acids levels were determined enzymatically using LabAssay Triglyceride and LabAssay NEFA, respectively, from Wako Pure Chemical Industries (Osaka, Japan). Serum leptin was measured using Mouse/Rat Leptin ELISA (BioVendor, Karásek, Czech Republic).
Arimochi H., Sasaki Y., Kitamura A, & Yasutomo K. (2016). Differentiation of preadipocytes and mature adipocytes requires PSMB8. Scientific Reports, 6, 26791.
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2

Plasma Hormone Levels and Osmolality

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Plasma leptin concentrations were determined in triplicate by a commercially available enzyme linked immunosorbent assay (ELISA) kit (mouse/rat leptin ELISA, BioVendor, Asheville, NC). Plasma corticosterone concentrations were determined in triplicate using a commercially available ELISA (Enzo Life Sciences, Farmingdale, NY). The detection limits were 50 pg/ml and 1.4 pg/ml, respectively. Plasma osmolality (Multi-Osmette, Precision Systems) was determined in plasma for 4 rats per diet in the food intake experiment. Hematocrit was determined in 7 rats per diet.
Pitynski-Miller D., Ross M., Schmill M., Schambow R., Fuller T., Flynn F.W, & Skinner D.C. (2017). A High Salt Diet Inhibits Obesity and Delays Puberty in the Female Rat. International journal of obesity (2005), 41(11), 1685-1692.
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3

Plasma Hormone Levels and Osmolality

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Plasma leptin concentrations were determined in triplicate by a commercially available enzyme linked immunosorbent assay (ELISA) kit (mouse/rat leptin ELISA, BioVendor, Asheville, NC). Plasma corticosterone concentrations were determined in triplicate using a commercially available ELISA (Enzo Life Sciences, Farmingdale, NY). The detection limits were 50 pg/ml and 1.4 pg/ml, respectively. Plasma osmolality (Multi-Osmette, Precision Systems) was determined in plasma for 4 rats per diet in the food intake experiment. Hematocrit was determined in 7 rats per diet.
Pitynski-Miller D., Ross M., Schmill M., Schambow R., Fuller T., Flynn F.W, & Skinner D.C. (2017). A High Salt Diet Inhibits Obesity and Delays Puberty in the Female Rat. International journal of obesity (2005), 41(11), 1685-1692.
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4

Fasting Mouse Plasma Lipid Analysis

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At 2:00 p.m., after 6 h fasting, submandibular vein blood (300 µL) was collected from conscious mice in tubes containing 5 U.I. of heparin (Choay heparin, Sanofi-aventis, Paris, France). Samples were centrifuged for 10 min at 1500× g at 20 °C, plasma collected and stored at −20 °C. For F0, total cholesterol level was measured by colorimetric dosage on Ortho Vitros Clinical Chemistry System instrument in Ambroise Paré Hospital (Boulogne-Billancourt, France). For F1, cholesterolemia was measured by enzymatic CHOD-PAP (cholesterol oxidase: p-aminophenazone) method (#WCHO100, Sobioda, Montbonnot-Saint-Martin, France). Leptinemia was measured with Leptin Mouse/Rat ELISA (#RD291001200R, BioVendor Laboratory Medicine, Brno, Czech Republic) according to the manufacturer’s guidelines.
Panchenko P.E., Lacroix M.C., Jouin M., Voisin S., Badonnel K., Lemaire M., Meunier N., Safi-Stibler S., Persuy M.A., Jouneau L., Durieux D., Lecoutre S., Jammes H., Rousseau-Ralliard D., Breton C., Junien C., Baly C, & Gabory A. (2019). Effect of Maternal Obesity and Preconceptional Weight Loss on Male and Female Offspring Metabolism and Olfactory Performance in Mice. Nutrients, 11(5), 948.
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5

Plasma Biomarkers in Metabolic Disorders

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Plasma LBP (HK205, LBP mouse ELISA kit, Hycult Biotech, PA, USA), insulin (0030-40-1, Mouse Insulin ELISA Kit, High Sensitivity, Quantitative, Life Technologies, Delhi, India), adiponectin (RD293023100R, Adiponectin Mouse ELISA, BioVendor - Laboratorni medicina, Brno, Czech Republic), leptin (RD291001200R, Leptin Mouse/Rat ELISA, BioVendor - Laboratorni medicina, Brno, Czech Republic), triglycerides (MAK266, MERCK LIFE SCIENCE, Madrid, Spain), WAT lipase activity (K723-100, Lipase Activity Colorimetric Assay kit II, Biovision, Inc., CA, USA), plasma free fatty acids (700310, Free Fatty Acid Fluorometric Assay Kit, Cayman chemical, MI, USA), plasma glycerol (10010755, Glycerol Colorimetric Assay Kit, Cayman chemical, MI, USA), and blood glucose (Accutrend; Roche Diagnostics, Mannheim, Germany) were measured using commercial kits according to manufacturer's instructions.
Latorre J., Ortega F., Oliveras-Cañellas N., Comas F., Lluch A., Gavaldà-Navarro A., Morón-Ros S., Ricart W., Villarroya F., Giralt M., Fernández-Real J.M, & Moreno-Navarrete J.M. (2022). Specific adipose tissue Lbp gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression. Molecular Therapy. Nucleic Acids, 27, 870-879.
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6

Insulin, NEFA, Leptin, and T4 Measurement

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Insulin concentration was measured using the Mouse/Rat Insulin kit (Meso Scale Discovery, Rockville, MD, USA). NEFA levels were measured using the NEFA-C kit (Wako Chemicals, Neuss, Germany). Leptin concentrations were measured with the leptin Mouse/Rat ELISA (BioVendor, RD291001200R, Heidelberg, Germany) and T4 concentrations with the T4 ELISA kit (Invitrogen, EIAT4C, Carlsbad, CA, USA).
Straub L.G., Efthymiou V., Grandl G., Balaz M., Challa T.D., Truscello L., Horvath C., Moser C., Rachamin Y., Arnold M., Sun W., Modica S, & Wolfrum C. (2019). Antioxidants protect against diabetes by improving glucose homeostasis in mouse models of inducible insulin resistance and obesity. Diabetologia, 62(11), 2094-2105.
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