Na fluor influenza na assay kit

All chemicals were purchased from Sigma-Aldrich unless otherwise specified. NA-Fluor^TM Influenza NA Assay Kit was purchased from Applied Biosystems. Viral RNA Extraction Kit was purchased from Qiagen. Absorbent pads (CFSP203000, cellulose fiber) and NC membrane (HF090MC100) were purchased from Millipore. A polyester conjugate pad was purchased from Boreda Biotech. Anti-influenza A virus nucleoprotein antibody were purchased from Abcam. Influenza virus strains, including A/California/07/2009 (H1N1), A/Puerto Rico/8/1934 (H1N1), and A/Brisbane/10/2007 (H3N2), were originally provided from the Korea Centers for Disease Control and Prevention, Korea. A/swine/Korea/GC0503/2005 (H1N1) and A/canine/Korea/MV1/2012 (H3N2) were kindly provided by Professor Dae-Sub Song (Korea University). The pH1N1/H275Y mutant virus (H275Y mutation A/Korea2785/2009 pdm: NCCP 42017) was obtained from the National Culture Collection for Pathogens (NCCP) operated by the Korea National Institute of Health. The titers of viruses were measured by an RT-PCR assay from Promega according to the manufacturer’s instructions.

Gold(III) chloride (HAuCl₄) solution and sodium citrate were purchased from Sigma-Aldrich (USA). An NA-Fluor^TM Influenza NA assay kit and a Viral RNA extraction kit were purchased from Applied Biosystems (USA) and Qiagen (Germany), respectively. Absorbent pads (CFSP203000, cellulose fiber) and nitrocellulose (NC) membrane (HF090MC100) related to lateral flow assay strips were purchased from Millipore (USA). A conjugate pad (polyester pad) was purchased from Boreda Biotech (Korea). Anti-influenza A virus nucleoprotein (NP) antibody and influenza A H1N1 neuraminidase/NA (H274Y mutation) as a Tamiflu-resistant NA protein were purchased from Abcam and Sinco Biological Inc., respectively. All other chemicals were of analytical grade and used without further purification. Pandemic H1N1 virus (A/California/07/2009) (WT) was provided by the BioNano Health Guard Research Center (H-GUARD). Pandemic H1N1/H275Y NA-mutant virus (H275Y mutation A/Korea2785/2009 pdm: NCCP 42017) (Tamiflu resistance) isolated from Gyeongsangnamdo in Korea was obtained from the National Culture Collection for Pathogens (NCCP), which is operated by the Korea Centers for Disease Control and Prevention. All virus titers were determined by real-time PCR using a GoTaq^® 1-Step RT-qPCR System (Promega, USA) according to the manufacturer’s instructions.