The serum biochemical reagents included ALT (LOT: 140121005), AST (LOT: 140221004), ALP (LOT: 140321002), TC (LOT: 141621013), TG (LOT: 141721003), LDL (142021004), and HDL (LOT. 142121006). TNF-a (E-EL-R2856-96T), IL-6 (E-EL-R0015-96T), and IL-1β (E-EL-R0012-96T) ELISA kits were purchased from Elabscience Biotechnology Co., Ltd. Anti-Caspase-8 antibody (Abcam, ab108333), anti-Caspase-3 antibody (Abcam, ab184787), anti-Cytochrome C antibody (Abcam, ab133504), and anti-IKK-β antibody (Abcam, ab124957) were purchased from Abcam. BCL2 monoclonal antibody (60178-1-1 g) and GAPDH (60004-1-Ig) antibody were obtained from Proteintech Group, Inc. NF-κB p65 antibody, phospho-NF-κB p65 antibody (LOT:17), a-SMA (CST, #19245), IκB-α antibody (CST, #4812), and phospho-IκB-α antibody (CST, #2859) were provided by Cell Signaling Technology, Inc. Chemiluminescence ECL Detection Kit (P90719) was obtained from Bobst Biotechnology.
Nf κb p65 antibody
Manufactured by Proteintech
Sourced in United Kingdom
The NF-κB p65 antibody is a primary antibody that recognizes the p65 subunit of the NF-κB transcription factor. NF-κB p65 is a critical regulator of genes involved in inflammation, immune response, and cell survival.
Automatically generated - may contain errors
Lab products found in correlation
Ab108333, by Abcam (1 mentions)
α sma, by Cell Signaling Technology (1 mentions)
Phospho nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Anti ikk β antibody, by Abcam (1 mentions)
Gapdh antibody 60004 1 ig, by Proteintech (1 mentions)
Iκbα antibody, by Cell Signaling Technology (1 mentions)
Phospho iκbα antibody, by Cell Signaling Technology (1 mentions)
Anti cytochrome c antibody, by Abcam (1 mentions)
Anti caspase 3 antibody, by Abcam (1 mentions)
Anti caspase 8 antibody, by Abcam (1 mentions)
Immunofluorescence microscopy, by Olympus (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Erk1 2 antibody, by Proteintech (1 mentions)
Caspase 3 antibody, by Proteintech (1 mentions)
Tlr4 antibody, by Proteintech (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Tunel kit, by Roche (1 mentions)
Lc3 antibody, by Proteintech (1 mentions)
Caspase 8 antibody, by Proteintech (1 mentions)
3 protocols using nf κb p65 antibody
1
Biochemical and Inflammatory Markers in Cellular Assays
2
Immunofluorescent Visualization of NF-κB
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Macrophages at a density of 5 × 103 per well were seeded in 12-well chamber slides. Cells were cultured overnight and treated as designated. Cells were fixed in cold 100% methanol for 30 min and permeabilized in 0.3% Triton ×100 (Solarbio, in PBS) for 15 min. Then non-specific binding was blocked by 3% BSA (Sigma, in PBS) incubation for 1 h at room tempreture. Subsequently, the primary NF-κB p65 antibody (Proteintech, 1:100) was applied overnigh at 4°C, followed by incubation with the secondary Alexa Fluor 488 conjugated antibody (Proteintech, 1:400) for 1 h at room tempreture. After nuclear staining with DAPI (Solarbio), the images were captured under a immunofluorescence microscopy (Olympus, Tokyo, Japan).
3
Apelin Signaling in LPS-Induced Inflammation
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Lipopolysaccharide (LPS) (Sigma Company, USA), apelin-13 (QRPRLS HKGPMPF, Abcam Company, UK), F13A (QRPRLS HKGPMPA, biobyt Company), anti-apelin antibody, anti-ERK (phospho) phosphorylated antibody, anti-NF-κB P65 (phospho) phosphorylated antibody (all purchased from Abcam Company, UK), ERK1/2 antibody, Caspase-3 antibody, Caspase-8 antibody, NF-κB P65 antibody, LC3 antibody, TLR4 antibody (all purchased from Proteintech Company, China), TNF-α and IL-6, apelin-13 ELISA kit (all purchased from Proteintech Company, China), RNA stationary solution RNA later (Thermo Company, USA), TUNEL kit (Roche Company, USA), Vevo 2100 Small Animal Ultrasound Imaging System (Vissonics, Canada).
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