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Imagequant tl ver 8

Manufactured by GE Healthcare
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The ImageQuant TL ver. 8.1 is a software package for analysis of gel-based quantitative data. It provides tools for image acquisition, processing, and analysis of various types of gels, blots, and microplates.

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Lab products found in correlation

Prism 6.0h, by GraphPad (1 mentions) Prism 9, by GraphPad (1 mentions)

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ImageQuant TL (449 citations) ImageQuant (354 citations) ImageQuant TL 8.1 software (78 citations)

2 protocols using imagequant tl ver 8

1

Quantification and Analysis of Cas6 Activity

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ImageQuant TL ver. 8.1 (General Electric) was used for quantification of all in vitro Cas6 cleavage, spacer acquisition, RT, and binding assays. Excel ver. 16.16 (Microsoft) was used to determine mean, median and standard deviation values. Prism 6.0h (Graphpad Software) was used for curve fitting of binding, RT, and Cas6 assays in order to determine koff and Kd values.
Mohr G., Silas S., Stamos J.L., Makarova K.S., Markham L.M., Yao J., Lucas-Elío P., Sanchez-Amat A., Fire A.Z., Koonin E.V, & Lambowitz A.M. (2018). A Reverse Transcriptase-Cas1 Fusion Protein Contains a Cas6 Domain Required for Both CRISPR RNA Biogenesis and RNA Spacer Acquisition. Molecular cell, 72(4), 700-714.e8.
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2

Quantitative Analysis of DNA Repair Mechanisms

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ImageQuant TL ver. 8.1 (General Electric) was used for quantitation of in vitro primer extension, terminal transferase, snap-back replication, and MMEJ assays. Excel ver. 16 (Microsoft) was used to determine mean, median, and standard deviation values. Prism 9.0 (Graphpad Software) was used for curve fitting of primer extension, terminal transferase, snap-back replication and MMEJ assays in order to determine kobs and Amplitude values and to calculate p values using a student’s unpaired t-test. Bedtools v2.29.2 was used to determine gene counts. R (v4.0.3) package DESeq2 was used to normalize gene counts, identify differentially expressed genes, and calculate Benjamini-Hochberg procedure adjusted p values. The R package goseq was used to identify enriched Gene Ontology (GO) terms in differentially expressed genes between KO and WT and to calculate their p values based on hypergeometric distribution.
Park S.K., Mohr G., Yao J., Russell R, & Lambowitz A.M. (2022). Group II Intron-like Reverse Transcriptases Function in Double-Strand Break Repair. Cell, 185(20), 3671-3688.e23.
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