Antibodies against Csk (#2109 and #4980), phospho-Src (Tyr 416) (#2101; used for detecting human Src pY419), phospho-Src (Tyr 527) (#2105; used for detecting human Src pY530), His-Tag (#2366) were purchased from Cell Signaling Technology. Anti-Csk antibody (#17720–1-AP) for immunoprecipitation was purchased from proteintech. Antibodies against GAPDH (#ab37168), SUMO1(#ab32058) were from Abcam. Anti-Flag (M2), anti-HA and anti-Myc antibodies were from Sigma-Aldrich. Protein A/G PLUS Agarose beads (#K0115) were obtained from Santa Cruz Biotechnology. Ni2+-NTA agarose beads were from Qiagen. Glutathione Sepharose 4B beads (#17–0756-01) were from GE Healthcare Life Sciences. Puromycin (#P8833) and EDTA-free Protease Inhibitor Cocktail were from Sigma-Aldrich.
Phospho src tyr 527
Manufactured by Cell Signaling Technology
Phospho-Src (Tyr 527) is an antibody that recognizes Src protein phosphorylated at tyrosine 527. It is used to detect and analyze the phosphorylation state of Src, a key signaling protein, in cellular and biochemical assays.
Automatically generated - may contain errors
Lab products found in correlation
Phospho src tyr416, by Cell Signaling Technology (2 mentions)
Edta free protease inhibitor cocktail, by Merck Group (1 mentions)
Anti myc antibody, by Merck Group (1 mentions)
Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions)
Ni2 nta agarose bead, by Qiagen (1 mentions)
Puromycin, by Merck Group (1 mentions)
Antibodies against gapdh, by Abcam (1 mentions)
Glutathione sepharose 4b beads, by GE Healthcare (1 mentions)
His tag, by Cell Signaling Technology (1 mentions)
17β estradiol e2, by Merck Group (1 mentions)
4 hydroxytamoxifen 4 oht, by Merck Group (1 mentions)
Total akt, by Cell Signaling Technology (1 mentions)
Phospho egfr tyr1068, by Cell Signaling Technology (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Total p27, by Cell Signaling Technology (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
Total erbb2, by Merck Group (1 mentions)
Total egfr, by Cell Signaling Technology (1 mentions)
Fulvestrant ici182780, by Bio-Techne (1 mentions)
2 protocols using phospho src tyr 527
1
Antibody Validation for Protein Interaction Studies
2
Immunoblotting Analysis of Signaling Proteins
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Primary antibodies against phospho-srctyr416, phospho-srctyr527, src, phospho-ERK1/2 (p42/44), phospho-p90rsk, phospho-AKTser473, total-AKT, cyclin D1, phospho-EGFRtyr1068, total-EGFR and total p27 were purchased from Cell Signalling; total-ER and phospho-p27ser10 from Santa Cruz; total ERK1/2 and Actin (Sigma Aldrich) and PgR (Novacastra). Total-ERBB2 and phospho-ERBB2Tyr1248 were purchased from Millipore. Secondary horseradish peroxidase antibodies were obtained from Dako. 17-β-estradiol (E2) and 4-hydroxytamoxifen (4-OHT) were purchased from Sigma-Aldrich, UK; fulvestrant (ICI182780) from Tocris Bioscience and dasatinib from Selleckchem.
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