Log In Sign up
The largest database of trusted experimental protocols

Anti igf 1rβ

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

Anti-IGF-1Rβ is a laboratory reagent used for the detection and analysis of the insulin-like growth factor 1 receptor beta (IGF-1Rβ) protein. It is a specific antibody that binds to and identifies the IGF-1Rβ protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Anti lamin b, by Santa Cruz Biotechnology (2 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Horseradish peroxidase, by GE Healthcare (1 mentions) Anti irβ, by Santa Cruz Biotechnology (1 mentions) Anti ar, by Cell Signaling Technology (1 mentions) Anti erg1 2 3, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Anti phospho stat3 y705, by Cell Signaling Technology (1 mentions) Anti phospho akt t308, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Anti tgfβ receptor 1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Anti egfr, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti igf 1rβ

1

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared and processed as previously described (32 (link)). Membranes were incubated overnight with the following primary antibodies: anti-IRβ, anti-IGF2BP3, anti-GAPDH, anti-IGF1Rβ, anti-Lamin B (Santa Cruz Biotechnology), anti-phospho-ERK 1/2, anti-ERK 1/2, anti-phospho-Akt (Ser473), and anti-Akt (Cell Signaling Technology, Beverly, MA, USA) antibodies. Anti-rabbit, anti-mouse (GE Healthcare, Little Chalfont, UK), and anti-goat (Santa Cruz Biotechnology) antibodies conjugated to horseradish peroxidase were used as secondary antibodies.
Mancarella C., Pasello M., Manara M.C., Toracchio L., Sciandra E.F., Picci P, & Scotlandi K. (2018). Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 Influences Sensitivity to Anti-IGF System Agents Through the Translational Regulation of IGF1R. Frontiers in Endocrinology, 9, 178.
+ Open protocol
+ Expand
2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared and processed as previously described [43 (link)]. Membranes were incubated overnight with the following primary antibodies: anti-IGF-1Rβ, anti-IRβ, anti-GAPDH, anti-LAMIN B, anti-ERG-1/2/3 (Santa Cruz Biotechnology); anti-AR (Cell Signaling Technology); anti-rabbit or anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare) were used as secondary antibodies.
Mancarella C., Casanova-Salas I., Calatrava A., Ventura S., Garofalo C., Rubio-Briones J., Magistroni V., Manara M.C., López-Guerrero J.A, & Scotlandi K. (2015). ERG deregulation induces IGF-1R expression in prostate cancer cells and affects sensitivity to anti-IGF-1R agents. Oncotarget, 6(18), 16611-16622.
+ Open protocol
+ Expand
3

Comprehensive Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-REP1, anti-EGFR, anti-c-Src, anti-IGF-1Rβ, horseradish peroxidase (HRP)-conjugated anti-rabbit IgG, and HRP-conjugated anti-mouse IgG antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-EGFR antibodies (Y845, Y1068, Y1086, and Y1173) were obtained from Invitrogen (Eugene, OR, USA). Anti-phospho-Src (Y416), anti-STAT3, anti-phospho-STAT3 (Y705), anti-TGF-β receptor I, anti-phospho-AKT (T308), cleaved caspase 3, SKP2, Survivin, Cyclin D1, ERK1/2, phospho-ERK1/2, and anti-PARP antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-GAPDH was purchased from Abfrontier (Seoul, Korea). Anti-β-actin antibody was purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA).
Yun U.J., Sung J.Y., Park S.Y., Ye S.K., Shim J., Lee J.S., Hibi M., Bae Y.K, & Kim Y.N. (2017). Oncogenic role of rab escort protein 1 through EGFR and STAT3 pathway. Cell Death & Disease, 8(2), e2621-.
+ Open protocol
+ Expand
4

Investigating IGF-1 Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant IGF-1 was from PeproTech (Rocky Hill, NJ). BMS-754807 was purchased from Active Biochem, PD98059 and LY294002 were from Merck, VE-821and OSI-906 were from Selleckchem. Customized siRNA Library was from Qiagen. Antibodies specific for phospho-H2AX (Rabbit, #9718), phospho-Chk1 (Rabbit, #2348s), Phospho-AKT (Rabbit, # 4060s), AKT (Mouse, #2920s), phospho-ERK (Rabbit, #4941), and phospho-IGF1R Y1135/1136 (Rabbit #3024) were from Cell Signalling Technology, Danvers, MA. Anti-Chk1 (Mouse, #sc-8408), Anti-IGF1Rβ (Rabbit, #sc-711) and anti-α-Tubulin (Mouse, #sc-23948) antibodies were from Santa Cruz Biotechnology, Santa Cruz, CA. Anti-β-Actin (Mouse, #A5441) antibody was from Sigma Aldrich. Anti-ERK2 (Mouse, #4696) antibody was from Millipore.
O'Flanagan C.H., O'shea S., Lyons A., Fogarty F.M., McCabe N., Kennedy R.D, & O'Connor R. (2016). IGF-1R inhibition sensitizes breast cancer cells to ATM-related kinase (ATR) inhibitor and cisplatin. Oncotarget, 7(35), 56826-56841.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!