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5 protocols using nandrolone decanoate

1

Neuroendocrine Compound Preparation

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil. Eticlopride hydrochloride (ETIC), serotonin hydrochloride (5HT) and arginine vasopressin (AVP) were purchased from Sigma Aldrich (St. Louis, MO). The ETIC and AVP were dissolved in 0.9% (wt/vol) normal saline, and the 5HT was dissolved in ddH2O.
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2

Anabolic Steroids Administration Protocol

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil (SO). Eticlopride hydrochloride (ETIC) and arginine vasopressin (AVP) were purchased from Sigma Aldrich (St. Louis, MO). The ETIC was dissolved in 0.9% (wt/vol) normal saline and the AVP was dissolved in ddH2O.
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3

Anabolic Hormones and Vasopressin Regulation

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil (SO). The neuropeptide arginine vasopressin (AVP) and the linear AVP V1A receptor antagonist (Manning Compound) [OH-Phaa-D-Tyr(Me)-Phe-Gln-Asn-Arg-Pro-Arg-NH2] were purchased from Sigma Aldrich (St. Louis, MO). Manning compound was dissolved in in 0.9% (wt/vol) normal saline and AVP was dissolved in ddH20.
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4

Anabolic Steroid and GABAergic Protocol

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc. (Newport, RI) and prepared in sesame oil. Muscimol and bicuculine were purchased from Sigma Aldrich (St. Louis, MO) and dissolved in 0.9% (wt/vol) normal saline.
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5

Anabolic Steroids Preparation and Storage

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Testosterone cypionate, 17α-methyltestosterone, and nandrolone decanoate were purchased from Steraloids (Newport, RI). Sesame oil vehicle was purchased from Sigma. A fresh cocktail of all the three AAS in Sesame oil vehicle was made every week.
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