All the chemicals were purchased from Sigma-Aldrich (St. Louis, MO). All restriction endonucleases, Phusion polymerase, Klenow polymerase and T4 DNA ligase were purchased from New England Biolabs (Ipswich, MA). Pure H2O2, methylmethane sulphonate (MMS), ciprofloxacin, kanamycin and hygromycin were purchased from Sigma-Aldrich (St. Louis, MO). RT-qPCR reagents, SYBR RT-qPCR master mix and cDNA synthesis kit were obtained from Bio-Rad Laboratories (Hercules, CA). The DNA oligonucleotides were synthesized by Sigma-Genosys (Bangalore, India) and [α-32P]ATP was purchased from the Board of Radiation and Isotope Technology (Hyderabad, India).
Methylmethane sulphonate
Manufactured by Merck Group
Sourced in United States
Methylmethane sulphonate is a chemical compound commonly used in laboratory settings. It serves as a methylating agent, primarily for research purposes. The core function of this product is to facilitate the introduction of methyl groups into various molecules, which is a crucial step in many chemical reactions and studies.
Automatically generated - may contain errors
Lab products found in correlation
Hygromycin, by Merck Group (1 mentions)
Klenow polymerase, by New England Biolabs (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Cdna synthesis kit, by Bio-Rad (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Phusion polymerase, by New England Biolabs (1 mentions)
Alamar blue, by Merck Group (1 mentions)
2 aminoanthracene, by Merck Group (1 mentions)
9 aminoacridine, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Cadmium chloride, by Sisco Research Laboratories (1 mentions)
Lead nitrate, by Sisco Research Laboratories (1 mentions)
Tris buffer, by Sisco Research Laboratories (1 mentions)
Nutrient broth, by HiMedia (1 mentions)
Nutrient agar, by HiMedia (1 mentions)
4 protocols using methylmethane sulphonate
1
Chemicals and Reagents Used in Research
2
Evaluating MMS Cytotoxicity in RNAi-Induced Parasites
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Parasites in which RNAi has been pre-induced for 2 days with tetracycline (1 μg ml−1), were incubated in a 96-well plate (200 μl per well) with sixfold range dilutions of methyl methanesulphonate (MMS; Sigma) in HMI-11, at a final concentration of 0.5–4 × 104 cells ml−1. After 2 days, cell viability was measured with AlamarBlue® (Sigma). Ten microlitres of Alamar Blue® was added per well followed by incubation at 37°C, 4 h and fluorescence was measured (530ex/590em nm). RNAi induction with tetracycline and selection with Hygromycin was maintained during the 2-day exposure to MMS. MMS concentration is expressed in parts per million (ppm) of volume.
3
Reverse Mutation Assay Protocol
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For reverse mutation assay, methyl methane sulphonate (MMS), sodium azide, Nitrofluorene and 2-aminoanthracene were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 9-Amino Acridine was purchased from Fluka – Sigma Aldrich (St. Louis, MO, USA). The Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 strain WP2uvrA were purchased from Molecular Toxicology Inc. (Boone, NC, USA). A rat liver S9 fraction induced by aroclor in male Sprague–Dawley rats was purchased from Molecular Toxicology Inc. (Boone, NC, USA). The chemicals and solvents used throughout the experiments were of analytical grade.
4
Onion Genotoxicity and Bioremediation
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A. cepa (onion) red variety was purchased from local market of Aligarh. Methyl methane sulphonate (MMS) was procured from Sigma–Aldrich, USA. Cadmium chloride, lead nitrate and Tris buffer were obtained from Sisco Research Laboratories (SRL). Acetocarmine, iron allum and ethanol were obtained from Bangalore Genei, India. Glacial acetic acid, N-butyl alcohol and mannitol were purchased from Qualigens, India. Nutrient agar and Nutrient broth were purchased from Hi-media, India. Aligarh wastewater (AWW) and Mathura refinery wastewater (RWW) samples were collected from industrial effluents of Aligarh and Mathura refinery, respectively. Escherichia coli K12 strains were a kind gift from Dr. Mary K. Berlyn, Yale University, USA.
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