Media containing secreted luciferase was harvested at 48 hours post transfection, unless otherwise noted. Media was diluted 1:5 in PBS and then luciferase activity was measured using the BioLux Cypridinia and Biolux Gaussia luciferase assay kits (New England Biolabs) on a Biotek Synergy 4 plate reader with an injection protocol. All replicates were performed as biological replicates.
Biolux gaussia luciferase assay
Manufactured by New England Biolabs
The BioLux Gaussia Luciferase Assay is a luminescence-based detection system for measuring the activity of the Gaussia luciferase reporter gene. The assay utilizes the Gaussia luciferase enzyme, which catalyzes the oxidation of its substrate to produce light, allowing for sensitive and quantitative detection of gene expression or other biological processes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using biolux gaussia luciferase assay
1
Secreted Luciferase Assay Protocol
2
Serological Neutralization Assay for Paramyxoviruses
3
Neutralizing Antibody Assay for Paramyxoviruses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The relevant bat and human serum samples were also tested for neutralizing antibodies using the replication-competent recombinant paramyxoviruses (rSeV-eGFP or rNiV-GLuc) generated as described above. SN of live rSeV-eGFP and rNiV-GLuc infection was performed in an identical manner as VSVpp (the latter under BSL-4 conditions), except that rSeV-eGFP infection was detected by FACS analysis and rNiV-GLuc infection was detected by quantifying GLuc activity (BioLux Gaussia Luciferase Assay, New England Biolabs) in 10% (v/v) of infected cell culture supernatant at 24 h.p.i.
FCS and hyperimmune rabbit anti-NiV serum53 (link) were used as negative and positive controls, respectively. Additional negative controls included NHS from Los Angeles blood donors. These anonymized and de-identified blood samples were obtained on a fee-for-service basis from the Virology Core at the UCLA AIDS Institute. Core services were approved by and consistent with all IRB policies at UCLA. NBS from captive-bred bats were generously provided by Brevard Zoo, Melbourne, Florida, USA. All SN assays were performed in quadruplicates.
FCS and hyperimmune rabbit anti-NiV serum53 (link) were used as negative and positive controls, respectively. Additional negative controls included NHS from Los Angeles blood donors. These anonymized and de-identified blood samples were obtained on a fee-for-service basis from the Virology Core at the UCLA AIDS Institute. Core services were approved by and consistent with all IRB policies at UCLA. NBS from captive-bred bats were generously provided by Brevard Zoo, Melbourne, Florida, USA. All SN assays were performed in quadruplicates.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!