Anti caspase 3 antibody

Example 3

Caspase-3 Activation after MV or MV-deltaC Infection

The caspase-3 activation was analysed both in tumour cells infected with unmodified MV and MV-deltaC (FIG. 7B). A panel of two epithelioid mesothelioma cell lines (Meso13 and Meso56), one melanoma cell line (M17) and one lung adenocarcinoma cell line (A549) were infected with unmodified MV or MV-deltaC, at a MOI of 1.0 for 2 hours, incubation at 37° C. Control cell lines were not infected with MV or MV-deltaC. Virus infected (MV or MV-deltaC) and uninfected tumour cells were analysed by flow cytometry after staining with an anti-caspase-3 antibody (BD Biosciences) three days after infection.

Infection with MV-deltaC induced caspase-3 activation for the two different tested cell lines: 38.2% for Meso13 epithelioid mesothelioma cells, 30.2% for M17 melanoma cells, 21.8% for A549 lung adenocarcinoma cells and 8.4% for Meso56 epithelioid mesothelioma cells. On the contrary, this caspase-3 activation was not or partially observed after infection with unmodified MV. These results suggested that unmodified MV and MV-deltaC viruses could induce the tumour cell death according to two different pathways.

Example 3

Caspase-3 Activation After MV or MV-DeltaC Infection

The caspase-3 activation was analysed both in tumour cells infected with unmodified MV and MV-deltaC (FIG. 7B). A panel of two epithelioid mesothelioma cell lines (Meso13 and Meso56), one melanoma cell line (M17) and one lung adenocarcinoma cell line (A549) were infected with unmodified MV or MV-deltaC, at a MOI of 1.0 for 2 hours, incubation at 37° C. Control cell lines were not infected with MV or MV-deltaC. Virus infected (MV or MV-deltaC) and uninfected tumour cells were analysed by flow cytometry after staining with an anti-caspase-3 antibody (BD Biosciences) three days after infection.

Infection with MV-deltaC induced caspase-3 activation for the two different tested cell lines: 38.2% for Meso13 epithelioid mesothelioma cells, 30.2% for M17 melanoma cells, 21.8% for A549 lung adenocarcinoma cells and 8.4% for Meso56 epithelioid mesothelioma cells. On the contrary, this caspase-3 activation was not or partially observed after infection with unmodified MV. These results suggested that unmodified MV and MV-deltaC viruses could induce the tumour cell death according to two different pathways.