Rabbit anti b9d1 antibody

Manufactured by Abnova

Rabbit anti-B9D1 antibody is a laboratory reagent used in research applications. It is an antibody raised in rabbits that specifically binds to the B9D1 protein. The core function of this antibody is to allow detection and study of the B9D1 protein in various experimental settings.

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Lab products found in correlation

Cy2 donkey anti mouse igg antibody, by Jackson ImmunoResearch (2 mentions) Mouse anti acetylated α tubulin antibody, by Merck Group (1 mentions) Mouse anti acetylated α tubulin, by Merck Group (1 mentions)

2 protocols using rabbit anti b9d1 antibody

Immunostaining of Embryonic Neural Tissues

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Published procedures were used for staining [30 (link), 31 ] and thick sectioning for the neural tube [32 (link)] with minor modifications. Embryos were fixed in MEMFA for either 2.5 h at room temperature or overnight at 4 °C for anti-acetylated-α-tubulin, anti-B9D1 and anti-γ-tubulin antibodies or in methanol for 48 h at 4 °C for anti-centrin antibody. Fixed embryos were dehydrated completely in methanol at −20 °C for at least several hours and rehydrated consecutively with PBS. After rinsing in PBT (0.1% Triton X-100 in PBS), embryos were incubated with 10% goat serum in PBT at room temperature for at least 1 h. Samples were incubated with mouse anti-acetylated-α-tubulin antibody (1:500, Sigma), rabbit anti-B9D1 antibody (1:50, abnova), rabbit anti-γ-tubulin antibody (1:50, abcam) and mouse anti-centrin antibody (1:50, Millipore) overnight at 4 °C. Primary antibodies were recognized with Cy2 donkey anti-mouse IgG antibody (1:500, Jackson ImmunoResearch) and Alexa Flour 648 goat anti-rabbit IgG antibody (1:500, Life Technologies), respectively. Antibodies were diluted in 10% goat serum in PBT. Images were taken by confocal microscopy.

Manojlovic Z., Earwood R., Kato A., Perez D., Cabrera O.A., Didier R., Megraw T.L., Stefanovic B, & Kato Y. (2017). La-related protein 6 controls ciliated cell differentiation. Cilia, 6, 4.

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Immunofluorescent Labeling of Cilia in Embryos

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Published procedures were used for staining (Lee et al., 2008 ; Suzuki et al., 2007 (link)) and thick sectioning (Becker and Gard, 2006 (link)) with minor modifications. Embryos were fixed in MEMFA for either 2.5 hours at room temperature or overnight at 4 °C. Fixed embryos were dehydrated completely in methanol at −20 °C for at least several hours and rehydrated consecutively with PBS. After rinsing in PBT (0.1% Triton X-100 in PBS), embryos were incubated with 10% goat serum in PBT at room temperature for at least 1 hour. Samples were incubated with mouse anti-acetylated α-tubulin (1:500, Sigma) antibody, rabbit anti-LRD antibody (1:10, gifted by Dr. M. Levin), rabbit anti-phospho-Smad2 antibody (Ser465/467) (1:20, Cell Signaling), rabbit anti-B9D1 antibody (1:50, abnova) and/or anti-γ-tubulin antibody (1:50, abcam) overnight at 4 °C. Primary antibodies were recognized with Cy2 donkey anti-mouse IgG antibody (1:500, Jackson ImmunoResearch), Alexa Flour 546 anti-rabbit IgG antibody (1:500, Life Technologies), or Cy5 donkey anti-rabbit IgG antibody (1:500, Jackson ImmunoResearch), respectively. Antibodies were diluted in 10% goat serum in PBT. Images were taken by confocal microscopy and cilia were measured by using ImageJ software.

Tözser J., Earwood R., Kato A., Brown J., Tanaka K., Didier R., Megraw T.L., Blum M, & Kato Y. (2015). TGF-β signaling regulates the differentiation of motile cilia. Cell reports, 11(7), 1000-1007.

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