Vectastain abc kit for immunohistochemistry

Anti-mouse F4/80 Ab (Cl:A3-1) and anti-TGF-β (AHP1734) was purchased from Serotec (Raleigh, NC). Anti-p50 (ab7971), anti-p65/RelA (ab7970), anti-iNOS (ab3523), and anti- TNF-α (ab 6671) were purchased from Abcam (Cambridge, Massachusetts). F4/80-FITC-conjugate (BM8), CD36-PE-conjugate (No.72-1) were purchased from eBioscience (San Diego, California). Anti- IL-1β Ab (AF-401-NA), recombinant mouse (rm) GM-CSF (rm GM-CSF), rm IL-4, and Duoset TNF-α ELISA kit were purchased from R&D Systems (Minneapolis, MN). Anti-phospho p65/RelA (3033) was purchased from Cell Signaling (Danvers, MA). Anti-Arg1 (sc-18354) was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX). Vectastain ABC kit for immunohistochemistry and Vectashield mounting medium was purchased from Vector Laboratories (Burlingame, CA). May-Grunwald and Wright-Giemsa stains were obtained from Sigma (St. Louis, Missouri). Filters (0.45-μm pore size, mixed cellulose ester) were acquired from Millipore (Billerica, MA) and PDMS from Invotec International (Jacksonville, FL). PDMS disks (4 mm in diameter and 1 mm thick) were cut with the aid of a biopsy punch (Acuderm; Fort Lauderdale, FL).

Recombinant human (rh) M-CSF, recombinant mouse (rm) IL-4, and rh FLT-3were purchased from R&D Systems (Minneapolis, MN). Vectastain ABC kit for immunohistochemistry and Vectashield mounting medium was purchased from Vector Laboratories (Burlingame, CA). May-Grunwald and Wright-Giemsa stains were obtained from Sigma (St. Louis, Missouri). Filters (0.45-μm pore size, mixed cellulose ester) were acquired from Millipore (Billerica, MA) GeneChip miRNA 2.0 Arrays and mouse Gene 1.0st Arrays were purchased from Affymetrix (Santa Clara, California). PLGA was obtained from Durect Corporation (Pelham, Alabama), spermidine and Coumarin 6 from Sigma-Aldrich (St. Louis, Missouri). A custom miRIDIAN mimic for mmu-miR-223-3p was purchased from Thermo Fisher Scientific Biosciences Inc. (Chicago, IL) and miRIDIAN mimic negative control #1 was purchased from GE Healthcare Dharmacon, Inc. (Lafayette, Colorado). Polydimethylsiloxane (PDMS) was purchased from Invotec International (Jacksonville, Florida) and was cut into 4mm diameter and 1mm thick discs using a biopsy punch from Acuderm (Fort Lauderdale, Florida).