Human Embryonal Carcinoma cell line NTERA-2 and the murine testicular cell lines TM4 (Sertoli), GC-1 (Spermatogonia), and GC-2 (Spermatocytes) were maintained in DMEM (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK) [25] , [26] (link), [27] (link), [28] (link). Olaparib (AZD2281) and P005091 were provided by SelleckChem. Cycloheximide, cisplatinum, H2O2 (H1009), erastin (E7781), ferrostatin-1 (SML0583) and desferoxamine (D9533) were from Sigma-Aldrich, Inc (St. Louis, CA, USA). Z-VAD-fmk (FMK001) was from MedChemExpress.
Ferrostatin 1 sml0583
Manufactured by Merck Group
Sourced in United States
Ferrostatin-1 (SML0583) is a chemical compound produced by Merck Group. It functions as a small molecule inhibitor. The core purpose of Ferrostatin-1 is to inhibit ferroptosis, a form of regulated cell death.
Automatically generated - may contain errors
Lab products found in correlation
Desferoxamine, by Merck Group (1 mentions)
Cis platinum, by Merck Group (1 mentions)
P005091, by Selleck Chemicals (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Ifsp1, by Merck Group (1 mentions)
Anti β actin a5441, by Merck Group (1 mentions)
Anti gsdmd 20770 1 ap, by Proteintech (1 mentions)
Anti hmgcr sc 271595, by Santa Cruz Biotechnology (1 mentions)
Normal rabbit igg, by Cell Signaling Technology (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
Rsl3 s8155, by Selleck Chemicals (1 mentions)
4 protocols using ferrostatin 1 sml0583
1
Maintenance of Embryonal and Testicular Cell Lines
2
Ferroptosis Inhibitor Screening Protocol
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Erastin (571203-78-6) and RSL3 (1219810-16-8) were bought from Tocris Bioscience (Bio-Techne GmbH, Wiesbaden, Germany). Ferrostatin-1 (SML0583), phenprocoumon (SML2365) and iFSP1 (SML2749) were purchased from Sigma-Aldrich. Brequinar (HY-108325) was purchased from MedChemExpress (via Hölzel Diagnostika, Cologne, Germany). Vitamin K1 (phytomenadione) dissolved at a concentration of 10 mg/ml in glycocholic acid, [3-sn-phosphatidyl]choline, and sodium hydroxide was obtained from CHELAPHARM Arzneimittel GmbH (Greifswald, Germany).
3
Ferroptosis Pathway Regulation Analysis
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The applied antibodies were as follows: anti‐BRCC36 (15391‐1‐AP), anti‐Ubiquitin (10201‐2‐AP), anti‐NLRP3 (19771‐1‐AP), and anti‐GSDMD (20770‐1‐AP) were purchased from Proteintech (Rosemont, Illinois, USA); anti‐HMGCR (sc‐271595) was purchased from Santa Cruz (Dallas, Texas, USA); anti‐cleaved‐GSDMD (#36 425), anti‐BRCC36 (#18215S), and Normal Rabbit IgG (#2729) were purchased from Cell Signaling Technology; anti‐HMGCR (ab174830), anti‐cleaved caspase‐1 (ab207802), and anti‐4‐Hydroxynonenal (ab46545) were purchased from Abcam. Anti‐β‐actin (A5441) and anti‐Flag (F1804) were purchased from Sigma‐Aldrich. Anti‐HMGCR (A19063) was purchased from ABclonal. The reagents used in the experiment were as follows: RSL3 (S8155) was obtained from Selleck; Ferrostatin‐1 (SML0583) and Lithium phosphorus sulfide (LPS) (L2630) were purchased from Sigma‐Aldrich; Thiolutin (THL) and 25‐hydroxycholesterol were obtained from APExBIO.
4
Ferroptosis Attenuates LPS-Induced AKI
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All procedures were conducted in accordance with the guidelines for animal care and use from the National Institutes of Health. Male C57BL/6 mice (aged 6–8 weeks and weighing 22–25 g) were obtained from the Experimental Animal Center, Sichuan Provincial People’s Hospital, and were fed a standard laboratory diet. LPS and ISL were dissolved in normal saline and 0.5% Tween-20/saline, respectively. AKI mice were developed by intraperitoneal (i.p.) LPS injection. A total of 30 mice were randomly divided into six groups (n = 5): control, ISL, Fer, LPS, LPS plus ISL, and LPS plus Fer. An intraperitoneal injection of LPS (10 mg/kg) was made to induce septic AKI. ISL was administered via gavage at 50 mg/kg 30 min before LPS injection. Mice were dosed intraperitoneally with Fer (Ferrostatin-1, SML0583, Sigma-Aldrich, St. Louis, MO) at 5 mg/kg. Mice were sacrificed by cervical dislocation 8 h after LPS injection. Kidney tissue and serum samples were collected concurrently.
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