Log In Sign up
The largest database of trusted experimental protocols

Fxr1 primary antibody

Manufactured by Proteintech
Visit Supplier

The FXR1 primary antibody is a research-use only product designed for the detection of FXR1 (Fragile X Mental Retardation Syndrome-Related Protein 1) in various sample types. This antibody is intended for use in applications such as Western blotting and immunohistochemistry to help researchers study the expression and localization of FXR1 in their experiments.

Automatically generated - may contain errors

Lab products found in correlation

Steamer set, by IHC World (2 mentions) Caseviewer software, by 3DHISTECH (2 mentions) Ihc tek epitope retrieval solution, by IHC World (2 mentions) Vector red alkaline phosphatase substrate kit 1, by Vector Laboratories (2 mentions) Harris modified hematoxylin, by Thermo Fisher Scientific (2 mentions) Cmyc primary antibody, by Santa Cruz Biotechnology (2 mentions) Vectastain abc ap kit, by Vector Laboratories (2 mentions) Panoramic 250 flash 3 scanner, by 3DHISTECH (2 mentions)

2 protocols using fxr1 primary antibody

1

Quantification of FXR1 and cMYC in Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
FXR1 and cMYC protein levels in human ovarian cancer tissues were analyzed using three TMAs (Cat# OV1005bt, Cat# OVC961 and Cat#OV1004, US Biomax Inc., Rockville, MD). For this purpose, the slides were dewaxed in xylene, and rehydrated through graded ethanol to distilled water. Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). The slides were then immersed in 3% H2O2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100. Following, Vector red staining, the slides were counterstained with Harris modified hematoxylin (Thermo Fisher Scientific Inc., Rockford, IL), dehydrated with graded ethanol and xylene, and finally mounted with paramount. TMAs slides was digitally scanned using Panoramic 250 FLASH III scanner (3D HISTECH ltd. Version 2.0) and, using the Case Viewer software (3D HISTECH ltd. Version 2.0) was used to view and analyze images.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand
2

Quantification of FXR1 and cMYC in Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
FXR1 and cMYC protein levels in human ovarian cancer tissues were analyzed using three TMAs (Cat# OV1005bt, Cat# OVC961 and Cat#OV1004, US Biomax Inc., Rockville, MD). For this purpose, the slides were dewaxed in xylene, and rehydrated through graded ethanol to distilled water. Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). The slides were then immersed in 3% H2O2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100. Following, Vector red staining, the slides were counterstained with Harris modified hematoxylin (Thermo Fisher Scientific Inc., Rockford, IL), dehydrated with graded ethanol and xylene, and finally mounted with paramount. TMAs slides was digitally scanned using Panoramic 250 FLASH III scanner (3D HISTECH ltd. Version 2.0) and, using the Case Viewer software (3D HISTECH ltd. Version 2.0) was used to view and analyze images.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!