The LC-MS/MS system consisted of a Thermo TSQ Quantum Access (Thermo-Fisher) electrospray ionization triple quadrupole mass spectrometer coupled to an Ultimate 3000 HPLC system (Dionex Corporation, Sunnyvale, CA). Chromatographic separations were achieved using a Luna C18 column (150 × 2 mm) and a mobile phase consisting of a gradient from 10% to 95% CH3CN.
Thermo tsq quantum access
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Thermo TSQ Quantum Access is a triple quadrupole mass spectrometer designed for sensitive and selective quantitative analysis. It features advanced electronics, high-performance ion optics, and a rugged design for reliable operation. The instrument provides fast scanning and high-resolution capabilities to support a wide range of analytical applications.
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2 protocols using thermo tsq quantum access
1
LC-MS/MS Analysis of Compounds
2
Quantitative LC-MS/MS Analysis
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Liquid Chromatography Mass Spectrometry/Mass Spectrometry (LC/MS/MS) was performed using a Thermo TSQ Quantum Access (Thermo-Fisher) Triple Quadrupole Mass Spectrometer at the Proteomics Center (Health Sciences Tower, Stony Brook University, NY, USA). Chromatographic separation was performed on Aquasil C18 column, with water and acetonitrile (ACN) at 200 ul/min. Detection was carried out using multiple reaction monitoring (MRM) m/z 426 > 262 and 426 > 289 (confirmation ion) using electrospray ionization in negative ion mode. Samples were prepared as follows: 50 ul sample + 150 uACN and the precipitated proteins removed by centrifugation. Then, 50 ul supernatant was mixed with 50 ul water and 10 ul injected for analysis.
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