Hemoglobin, hematocrit, platelet count, and WBC were analyzed on a Cell-Dyn Sapphire analyzer (Abbot Diagnostics, Abbot Park, IL). The
Leucocount kit from BD (BD Biosciences, San Jose, CA) was used for residual WBC analysis, with a
BD FACSCanto II cytometer. Plasma hemoglobin was analyzed with the
HemoCue Plasma/Low Hb photometer (HemoCue AB, Angelholm, Sweden) to calculate hemolysis.
Lactate and blood gas analysis was performed on whole blood samples with an ABL 800 FLEX (Radiometer Medical ApS, Brøndshøj, Denmark).
Glucose, potassium, and Factor VIII were analyzed with the
Cobas 8000 ISE module (Roche Diagnostics GmbH, Rotkreuz, Switzerland). FVIII and fibrinogen levels were analyzed with the STA-R Evolution/STA-R Max platform (Stago S.A.S., Asnieres-sur-Seine, Paris, France).
All plasma samples were prepared by centrifugation at 1850 G for 10 min in RT.
Lunde T.H.F., Hartson L., Bailey S.L, & Hervig T.A. (2021). In vitro characteristics and in vivo platelet quality of whole blood treated with riboflavin and UVA/UVB light and stored for 24 hours at room temperature. Transfusion, 61 Suppl 1.