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Mops sodium dodecyl sulfate running buffer

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MOPS sodium dodecyl sulfate running buffer is a laboratory reagent used for electrophoresis, specifically for the separation and analysis of proteins. It provides a consistent pH environment and ionic strength to facilitate the migration of proteins through a polyacrylamide gel.

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Lab products found in correlation

Ecl western blotting detection reagent, by Cytiva (4 mentions) Rabbit anti p smad2, by Merck Group (2 mentions) Rabbit anti actin, by Merck Group (2 mentions) Rabbit anti bcl 2, by Santa Cruz Biotechnology (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Rabbit anti egfp, by Thermo Fisher Scientific (2 mentions) Mouse anti ha, by Fortrea (2 mentions) Rabbit anti p jnk, by Cell Signaling Technology (2 mentions) Rabbit anti smad2, by Cell Signaling Technology (2 mentions) Rabbit anti bax, by Santa Cruz Biotechnology (2 mentions) Rabbit anti alk5, by Abcam (2 mentions) Mouse anti nse, by Thermo Fisher Scientific (2 mentions) Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Avantor (2 mentions) Bis tris gel, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor, by Merck Group (2 mentions) P smad2, by Merck Group (1 mentions) Smad2, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

MOPS running buffer (169 citations) MOPS buffer (158 citations) Sodium sulfate (47 citations) Running Buffer (9 citations)

4 protocols using mops sodium dodecyl sulfate running buffer

1

Hippocampal Protein Expression Analysis

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Hippocampi dissected from hemi-brains of mice were homogenized and lysed. After centrifugation at 13,000 g, protein concentrations were measured using the BCA protein assay kit (Pierce) and lysates were separated on a 4 to 12% Bis-Tris gels (Invitrogen) using MOPS sodium dodecyl sulfate running buffer (Invitrogen), electroblotted onto PVDF membranes (Millipore), and analyzed with rabbit anti-p-Smad2 (1:1000; Chemicon, AB3849), rabbit anti-Smad2 (1:1000; Cell Signaling Technology, 3102S), mouse anti-HA (1:1000; Covance, MMS-101P), rabbit anti-ALK5 (1:200, Abcam, AB31013), rabbit anti-Bcl-2 (1:1000, Santa Cruz, sc-492), rabbit anti-Bax (1:1000, Santa Cruz, sc-526), mouse anti-NSE (1:1000, Thermo Scientific, AMPA6696) rabbit anti-p-JNK (1:1000, Cell Signaling Technology, 9251S), rabbit anti-eGFP (1:1000, Life Technologies, A11122), and rabbit anti-actin (1:5000, Sigma, A-5060) antibodies. Signal intensities were detected using ECL Western blotting detection reagents (Amersham Biosciences).
He Y., Zhang H., Yung A., Villeda S.A., Jaeger P.A., Olayiwola O., Fainberg N, & Wyss-Coray T. (2014). ALK5-dependent TGF-β signaling is a major determinant of late stage adult neurogenesis. Nature neuroscience, 17(7), 943-952.
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2

Western Blot Analysis of p-Smad2 and Smad2

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Cells were homogenized and lysed using RIPA buffer (Amresco) with protease and phosphatase inhibitors (Sigma and Roche, respectively). After centrifugation at 13,000g for 5 min, protein concentrations were measured using the BCA protein assay kit (Pierce) and lysates were separated on a 4–12% Bis–Tris gels (Invitrogen) using MOPS sodium dodecyl sulfate running buffer (Invitrogen). Proteins were transferred with the iBlot system onto nitrocellulose membranes (Novex) and incubated with antibodies p-Smad2 (1:1000, Millipore) and Smad2 (1:1000, Cell Signaling Technology). Signal intensities were detected using ECL Western blotting detection reagents (Amersham Biosciences) and evaluated by ImageJ.
He Y., Yao X., Taylor N., Bai Y., Lovenberg T, & Bhattacharya A. (2018). RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells. Journal of Neuroinflammation, 15, 153.
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3

Western Blot Analysis of Signaling Proteins

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Cells were homogenized and lysed using RIPA buffer (Amresco) with protease and phosphatase inhibitors (Sigma and Roche, respectively). After centrifugation at 13,000g, protein concentrations were measured using the BCA protein assay kit (Pierce) and lysates were separated on a 4–12% Bis-Tris gels (Invitrogen) using MOPS sodium dodecyl sulfate running buffer (Invitrogen). Proteins were transferred with the iBlot system onto nitrocellulose membranes (Novex) and incubated with antibodies p-AKT (1:1000, Cell Signaling Technology), p-ERK (1:1000, Cell Signaling Technology), AKT (1:1000, Cell Signaling Technology), ERK (1:1000, Cell Signaling Technology), and GAPDH (1:1000, Millipore). Signal intensities were detected using ECL western blotting detection reagents (Amersham Biosciences) and evaluated by ImageJ.
He Y., Taylor N., Fourgeaud L, & Bhattacharya A. (2017). The role of microglial P2X7: modulation of cell death and cytokine release. Journal of Neuroinflammation, 14, 135.
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4

Hippocampal Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hippocampi dissected from hemi-brains of mice were homogenized and lysed. After centrifugation at 13,000 g, protein concentrations were measured using the BCA protein assay kit (Pierce) and lysates were separated on a 4 to 12% Bis-Tris gels (Invitrogen) using MOPS sodium dodecyl sulfate running buffer (Invitrogen), electroblotted onto PVDF membranes (Millipore), and analyzed with rabbit anti-p-Smad2 (1:1000; Chemicon, AB3849), rabbit anti-Smad2 (1:1000; Cell Signaling Technology, 3102S), mouse anti-HA (1:1000; Covance, MMS-101P), rabbit anti-ALK5 (1:200, Abcam, AB31013), rabbit anti-Bcl-2 (1:1000, Santa Cruz, sc-492), rabbit anti-Bax (1:1000, Santa Cruz, sc-526), mouse anti-NSE (1:1000, Thermo Scientific, AMPA6696) rabbit anti-p-JNK (1:1000, Cell Signaling Technology, 9251S), rabbit anti-eGFP (1:1000, Life Technologies, A11122), and rabbit anti-actin (1:5000, Sigma, A-5060) antibodies. Signal intensities were detected using ECL Western blotting detection reagents (Amersham Biosciences).
He Y., Zhang H., Yung A., Villeda S.A., Jaeger P.A., Olayiwola O., Fainberg N, & Wyss-Coray T. (2014). ALK5-dependent TGF-β signaling is a major determinant of late stage adult neurogenesis. Nature neuroscience, 17(7), 943-952.
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