Etc 273t water cooled peltier thermostated cell holder

Example 27

A thermoresponsivity test of compound 8 in 150 mM KCl was performed by the following procedure. A JASCO FP-6500 spectrofluorometer was used, and using ultrapure water obtained from a Milli-Q reagent system made in Millipore Corporation, KCl purchased from Wako Pure Chemical Industries, Ltd. dissolved to make a 150 mM concentration was used as a solvent. The initial concentration of the compound in this experiment was 0.01 w/v %, and the excitation wavelength was 456 nm. A JASCO ETC-273T water-cooled Peltier thermostated cell holder was used for temperature control of the solution, and temperature was measured with its accompanying thermocouple. Solution temperature was raised in steps of 2° C., and the fluorescence intensity at each temperature was measured.

The test results are shown in FIG. 19. Even when VBTMA was used as a cationic unit, change in fluorescence intensity in response to temperature increase in a 150 mM potassium chloride solution could be confirmed. With regard to temperature responsivity, it is desirable that acrylamide, methacrylamide, or acrylic acid is contained in all units, however, it was revealed that if the mole fraction in a copolymer is about 10% as in this example, the probe functions as a probe that well responds to temperature even when a usual vinyl-type monomer such as styrene group is comprised in a unit.

Example 27

A thermoresponsivity test of compound 8 in 150 mM KCl was performed by the following procedure. A JASCO FP-6500 spectrofluorometer was used, and using ultrapure water obtained from a Milli-Q reagent system made in Millipore Corporation, KCl purchased from Wako Pure Chemical Industries, Ltd. dissolved to make a 150 mM concentration was used as a solvent. The initial concentration of the compound in this experiment was 0.01 w/v %, and the excitation wavelength was 456 nm. A JASCO ETC-273T water-cooled Peltier thermostated cell holder was used for temperature control of the solution, and temperature was measured with its accompanying thermocouple. Solution temperature was raised in steps of 2° C., and the fluorescence intensity at each temperature was measured.

The test results are shown in FIG. 19. Even when VBTMA was used as a cationic unit, change in fluorescence intensity in response to temperature increase in a 150 mM potassium chloride solution could be confirmed. With regard to temperature responsivity, it is desirable that acrylamide, methacrylamide, or acrylic acid is contained in all units, however, it was revealed that if the mole fraction in a copolymer is about 10% as in this example, the probe functions as a probe that well responds to temperature even when a usual vinyl-type monomer such as styrene group is comprised in a unit.

Example 27

A thermoresponsivity test of compound 8 in 150 mM KCl was performed by the following procedure. A JASCO FP-6500 spectrofluorimeter was used, and using ultrapure water obtained from a Milli-Q reagent system made in Millipore Corporation, KCl purchased from Wako Pure Chemical Industries, Ltd. dissolved to make a 150 mM concentration was used as a solvent. The initial concentration of the compound in this experiment was 0.01 w/v %, and the excitation wavelength was 456 nm. A JASCO ETC-273T water-cooled Peltier thermostated cell holder was used for temperature control of the solution, and temperature was measured with its accompanying thermocouple. Solution temperature was raised in steps of 2° C., and the fluorescence intensity at each temperature was measured.

The test results are shown in FIG. 19. Even when VBTMA was used as a cationic unit, change in fluorescence intensity in response to temperature increase in a 150 mM potassium chloride solution could be confirmed. With regard to temperature responsivity, it is desirable that acrylamide, methacrylamide, or acrylic acid is contained in all units, however, it was revealed that if the mole fraction in a copolymer is about 10% as in this example, the probe functions as a probe that well responds to temperature even when a usual vinyl-type monomer such as styrene group is comprised in a unit.