All chemicals (reagent grade) used were purchased from Sigma–Aldrich (USA) and Sinopharm Chemical Reagent Co. Ltd. (China).1H NMR spectra were measured on Varian Unity Inova 300 or 400 MHz NMR Spectrometers at 25 °C and referenced to TMS. Chemical shifts are reported in ppm using the residual solvent line as internal standard. Splitting patterns are designed as s, singlet; d, doublet; t, triplet; m, multiplet. HRMS spectra were acquired on an Agilent Technologies 6220 Accurate-Mass TOF LC/MS. Analytical thin-layer chromatography (TLC) was performed on the glass-backed silica gel sheets (silica gel 60 Å GF254). All compounds were detected using UV light (254 or 365 nm). Analytical HPLC was conducted on SHIMADZU LC-20AD. Prior to Ki measurement, all compounds were determined to be >95% pure by HPLC based on the peak area percentage.
6220 accurate mass tof lc ms
Manufactured by Agilent Technologies
The Agilent 6220 Accurate-Mass TOF LC/MS is a high-performance time-of-flight mass spectrometer designed for liquid chromatography applications. It provides accurate mass measurements to enable precise compound identification and quantification.
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Lab products found in correlation
2 protocols using 6220 accurate mass tof lc ms
1
NMR and HRMS Characterization of Compounds
2
High-Resolution Mass Spectrometry Protocol
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High resolution mass spectra were recorded using a 6220 accurate-mass TOF LC/MS (Agilent). A Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm particle size) was used for the LC separation. The same solvents as for the HPLC-MS were used, and a linear gradient from 0 to 98% B over 4 min. was employed. The mass spectrometer was externally calibrated using Agilent tuning mix prior to measurement.
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